甲醛对雌性大鼠卵巢储备功能的影响

doi:10.3969/j.issn.1004-616x.2010.01.008

癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (1): 32-0034.doi: 10.3969/j.issn.1004-616x.2010.01.008

甲醛对雌性大鼠卵巢储备功能的影响

彭国庆1;2;钟才高1;张琼2;关岚1;余剑英2;肖芳1

1. 中南大学公共卫生学院, 湖南长沙 410078; 2. 中南大学湘雅医院，湖南长沙 410008

收稿日期:2009-07-23 修回日期:2009-09-22 出版日期:2010-01-30 发布日期:2010-01-30
通讯作者: 钟才高

Effects of formaldehyde on ovarian reserve function in female rats

PENG Guo-qing1;2; ZHONG Cai-gao1; ZHANG Qiong2;GUAN Lan1; YU Jian-ying2; XIAO Fang1

1. College of Public Health, Central South University， Changsha 410078, Hu'nan; 2. Xiangya Hospital,Central South University, Changsha 410078, Hu'nan, China

Received:2009-07-23 Revised:2009-09-22 Online:2010-01-30 Published:2010-01-30
Contact: ZHONG Cai-gao

摘要/Abstract

摘要： 目的：在亚慢性染毒的条件下，研究甲醛对SD雌性大鼠卵巢储备功能的影响。方法：将40只SD雌性大鼠随机分为正常对照组和3个不同浓度(0.2、2.0、20.0 mg/kg)的甲醛染毒组。腹腔注射甲醛染毒，每天1次，连续染毒14 d，14 d后称体质量，并采用股动脉放血处死受试动物，取血清，用化学发光法测定血清雌二醇(E2)浓度；用酶联免疫方法测定抑制素B(inhibin B)浓度；用放射免疫方法测定卵泡刺激素(FSH)和黄体生成激素(LH)的浓度。并解剖摘取卵巢称重，计算脏器系数。结果：甲醛染毒组与对照组比较，各染毒剂量组大鼠血清E2、Inhibin B水平明显降低(P<0.05)，FSH水平显著升高(P<0.05)；高、中剂量组大鼠血清LH水平均有所升高，但差异无统计学意义(P>0.05)。各染毒剂量组卵巢脏器系数显著降低(P<0.05)。结论：在亚慢性染毒条件下，甲醛对SD雌性大鼠卵巢储备功能有一定损害作用。

关键词: 甲醛, 卵巢储备功能, 雌二醇, 卵泡刺激素, 黄体生成激素, 抑制素B

Abstract: OBJECTIVE: To explore the effects of subchronic exposure to formaldehyde on ovarian reserve function in female rats. METHODS: Forty female Sprague-Dawley (SD) rats were randomly subdivided into a control group and three formaldehyde groups. The rats in formaldehyde groups were intraperitoneally treated with formaldehyde at 0.2, 2.0 and 20.0 mg/(kg•d)for 14 days. Radioimmunoassay was used to detect estradiol(E2), luteinizing hormone (LH), follicle stimulating hormone (FSH) and Inhibin B. Ovary and body weights of female rats were measured and the organic coefficient of ovary was counted. RESULTS: Compared with the control group, the levels of serum estradiol (E2) and Inhibin B in the groups treated with formaldehyde decreased significantly (P<0.05). The level of serum FSH increased and the organic coefficient of ovary decreased significantly(P<0.05), while the levels of LH didn't change obviously(P>0.05). CONCLUSION:The ovarian reserve function of female rats can be impaired after subchronically exposed to formaldehyde for 14 days.

Key words: formaldehyde, ovarian reserve function, estradiol E2, follicle stimulating hormone, luteinizing hormone, inhibin B

中图分类号:

R994.3

彭国庆, 钟才高, 张琼, 关岚, 余剑英, 肖芳. 甲醛对雌性大鼠卵巢储备功能的影响[J]. 癌变·畸变·突变, 2010, 22(1): 32-0034.

PENG Guo-qing, ZHONG Cai-gao, ZHANG Qiong, GUAN Lan, YU Jian-ying, XIAO Fang. Effects of formaldehyde on ovarian reserve function in female rats[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2010, 22(1): 32-0034.

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甲醛对雌性大鼠卵巢储备功能的影响

Effects of formaldehyde on ovarian reserve function in female rats

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