癌变·畸变·突变 ›› 1999, Vol. 11 ›› Issue (1): 14-015.doi: 10.3969/j.issn.1004-616x.1999.01.005

• 论著 • 上一篇    下一篇

两种新型杀虫剂对蚯蚓精子形态的影响

孔志明 臧 宇 沈进萍 罗 屿 钟 远   

  1. 南京大学污染控制与资源化研究国家重点实验室 南京大学环境科学与工程系 南京 210093
  • 收稿日期:1998-05-14 修回日期:1998-09-29 出版日期:1999-01-30 发布日期:1999-01-30

EFFECT OF TWO NEW PESTICIDES ON SPERM MORPHOLOGY OF EARTHWORMS ( EIS EN IA FO ETIDA )

Kong Zhiming, Zang Yu, Shen J inping, Luo Yu, Zhong Yuan   

  1. State Key Laboratory of Pollution Control and Resource Reuse, Department of Environmental Science and Engineering, Nanjing University, Nanjing 210093
  • Received:1998-05-14 Revised:1998-09-29 Online:1999-01-30 Published:1999-01-30

摘要: 本文提出了一种检测及评价土壤生态系统中遗传毒性的新方法。赤子爱胜蚓( Eisenia f oetida) 经人工土壤法染毒,暴露于不同剂量的两种新型杀虫剂———呲虫啉和抑食肼中,观察它们对蚯蚓精子形态的影响。实验结果表明,呲虫啉在浓度大于0. 5mg/ kg 干土时对蚯蚓精子畸变率有明显影响( P < 0. 01) ,并呈现剂量效应关系;而抑食肼在浓度高达100mg/ kg 干土时,与对照组相比才有显著差异( P < 0. 05) 。

关键词: 呲虫啉, 抑食肼, 赤子爱胜蚓, 精子形态试验

Abstract: In this paper, a new method to assess the environmental safety of pesticide has been proposed in terms of sperm deformity of the earthworm ( Eisenia f oetida) . The result s showed that the main sperm morphological deformity appeared to be shape changes of the sperm heads. Significant induction of sperm deformity ( P< 0. 01) and dose2effect relationship displayed when the earthworms were exposed to imidacloprid at the concent rations of more than 0. 5mg/ kg dry soil. However , The sperm deformity f requency of RH - 5849 - exposed groups did not show significant difference ( P < 0. 05) f rom the negative cont rol until the dose reached 100 mg/kg dry soil. This study suggest s that the detection of sperm abnormality in earthworms could provide an early warning system of polluted environment .

Key words: Imidacloprid, RH - 5849, Eisenia foetida, Sperm deformity test