癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (6): 417-419.doi: 10.3969/j.issn.1004-616x.2006.06.001

• 论著 •    下一篇

甲基硝基亚硝基胍诱导CHL细胞中γH2AX焦点的形成

石　为1,2；余艳柯2；朱心强2；杨　军2   

  1. 1浙江大学医学院病理与病理生理学教研室；2浙江大学公共卫生学院毒理学研究所
  • 收稿日期:2006-04-18 修回日期:2006-07-11 出版日期:2006-11-30 发布日期:2006-11-30
  • 通讯作者: 石　为

N-methyl-N-nitro-N-nitrosoguanidine Induces the Phosphorylation of Histone H2AX in CHL Cells

SHI Wei , YU Yan-ke, ZHU Xin-qiang , YANG Jun   

  1. (1.Department of Pathology ＆ Pathophysiology, Zhejiang University, School of Medicine; 2. Institute of Toxicology, Zhejiang University, School of Public Health Hangzhou, Zhejiang 310058, China)
  • Received:2006-04-18 Revised:2006-07-11 Online:2006-11-30 Published:2006-11-30
  • Contact: SHI Wei

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摘要： 背景与目的： 研究甲基硝基亚硝基胍(MNNG)是否可诱导中国仓鼠肺成纤维细胞(CHL)中γH2AX焦点的形成，以及是否有细胞系依赖性。 材料与方法： 应用MTT试验检测不同浓度MNNG对鼠CHL细胞的生存率的影响;应用免疫荧光实验检测细胞中γH2AX焦点的形成。 结果： MTT检测表明MNNG对CHL细胞的细胞毒性有明显的时间和剂量效应；免疫荧光实验发现1 mg/L MNNG处理2 h、8 h、24 h的CHL细胞中含有γH2AX焦点的细胞比例及焦点数均较对照组显著增加(P<0.05)，含有超过30个γH2AX焦点的细胞比例分别达到56％，92％和91％。 结论： MNNG可以诱导CHL细胞中γH2AX焦点的形成，并且有明显的时间效应。

关键词: MNNG, γH2AX, DNA损伤, 遗传毒物

Abstract: BACKGROUND＆AIM: To study whether N-methyl-N＇-nitro-N-nitrosoguanidine(MNNG) could induce the formation of γH2AX foci in CHL cells. MATERIALS AND METHODS: The cytotoxic effects of different concentrations of MNNG were evaluated by MTT test. The phosphorylation of H2AX was assessed by using immunofluorescent microscopy. RESULTS: MNNG exhibited a dose- and time-dependent cytotoxic effect on CHL cells. MNNG treatment(1 mg/L) significantly increased the ratio of γH2AX-positive cells and the number of foci/cell at 2, 8, and 24 h. The ratios of cells with over 30 foci/cell were about 56％,92％ and 91％, respectively. CONCLUSION: MNNG could induce the phosphorylation of H2AX in CHL cells in a time-dependent manner.

Key words: MNNG, γH2AX, DNA damage, genotoxin

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