结直肠癌组织中葡糖醛酸转移酶2B同工酶和肝细胞核因子的表达研究

doi:10.3969/j.issn.1004-616x.2008.05.005

癌变·畸变·突变 ›› 2008, Vol. 20 ›› Issue (5): 354-358.doi: 10.3969/j.issn.1004-616x.2008.05.005

结直肠癌组织中葡糖醛酸转移酶2B同工酶和肝细胞核因子的表达研究

张　燕/ 李延青/ 张尚忠/ 杨晓云/ 袁俊华/ 郭玉婷/ 卢雪峰

山东大学齐鲁医院消化内科,山东　济南　250012

收稿日期:2007-10-15 修回日期:2008-03-10 出版日期:2008-09-30 发布日期:2008-09-30

Expression of UDP_glucuronosyl_transferase 2B and Hepatic Nuclear Factor 1α in Colorectal Cancer Tissues

ZHANG Yan, LI Yan_qing, ZHANG Shang_zhong, YANG Xiao_yun, YUAN Jun_hua, GUO Yu_ting, LU Xue_feng

Department of Gastroenterology of Qilu Hospital, Shangdong University, Jinan 250012, Shangdong,China

Received:2007-10-15 Revised:2008-03-10 Online:2008-09-30 Published:2008-09-30

摘要/Abstract

摘要： 背景与目的：检测葡糖醛酸转移酶2B家族(UDP_glucuronosyltransferase 2B,UGT2B)4种同工酶及肝细胞核因子(hepatic nuclear factor1α,HNF1α)在结直肠癌组织中的表达，探讨其在结直肠癌变中的意义。材料与方法：选择32例结直肠癌组织、癌旁组织并选择14例正常人群结直肠黏膜为对照组，用RT_PCR方法检测UGT2B中 4种同工酶及肝细胞核因子HNF1α mRNA的表达。分别用Western blot方法检测UGT2B7蛋白，免疫组织化学方法检测HNF1α蛋白在结直肠癌组织、癌旁组织及正常结直肠粘黏膜内的表达。结果： UGT2B同工酶在结直肠黏膜内呈多态性表达。UGT2B10表达丰度及阳性表达率较低，而结直肠癌组织、癌旁组织中UGT2B4 mRNA水平与对照组相比无明显改变(P>0.05)，UGT2B7、UGT2B15 mRNA表达水平与对照组相比降低(P<0.05)；结直肠癌组织、癌旁组织UGT2B7蛋白荧光强度、蛋白灰度值比值低于正常结肠黏膜(P<0.05)。HNF1α mRNA及其蛋白的表达与对照组相比均无明显改变(P均>0.05)。结论： UGT2B同工酶表达降低可能是结直肠癌变的机制之一。UGT基因的上游调控因子HNF1α mRNA水平、蛋白水平表达无明显改变，可能与机体内更加复杂的调控机制有关。

关键词: 结直肠癌, 葡糖醛酸转移酶, 肝细胞核因子

Abstract: BACKGROUND AND AIM: To evaluate the expression of UDP_ glucuronosyltransferase (UGT) 2B subfamily and hepatic nuclear factor1α(HNF1α)in colorectal cancer, and to explore its significance in colorectal tissue carcinogenesis. MATERIALS AND METHODS: RT_PCR was used to determine the mRNA levels of four UGT isoforms and hepatic nuclear factor1α(HNF1α) in 32 samples of colorectal cancer and 14 normal colorectal sambles.UGT2B protein was measured by western blot analysis and immunochemistry was used for HNF1α protein. RESULTS: Polymorphic regulation of UGT2B isoforms expression was found in colorectal tissue; compared to control group, UGT2B4 mRNA level of the 32 samples of colorectal cancer and its surrounding healthy tissue was not significantly different(P>0.05). UGT2B7,UGT2B15 mRNA level were significantly down_regulated in colorectal cancer and its surrounding healthy tissues when compared to control group(P<0.05). UGT2B7 protein level was significantly decreased in colorectal cancer and its surrounding healthy tissues(P<0.05). Compared to control group, mRNA and protein levels of HNF1α were not significantly changed in colorectal cancer and its surrounding healthy tissues(P>0.05). CONCLUSION: Decreased expression level of UGT isoform may be one cause of colorectal tissue carcinogenesis. As an upstream regulator of UGT genes, mRNA and protein levels of HNF1α were not significantly changed. This may be related to complicated control mechanisms in vivo.

Key words: colorectal cancer, UDP_glucuronosyltransferase, hepatic nuclear factor1α

张　燕/ 李延青/ 张尚忠/ 杨晓云/ 袁俊华/ 郭玉婷/ 卢雪峰. 结直肠癌组织中葡糖醛酸转移酶2B同工酶和肝细胞核因子的表达研究[J]. 癌变·畸变·突变, 2008, 20(5): 354-358.

ZHANG Yan, LI Yan_qing, ZHANG Shang_zhong, YANG Xiao_yun, YUAN Jun_hua, GUO Yu_ting, LU Xue_feng. Expression of UDP_glucuronosyl_transferase 2B and Hepatic Nuclear Factor 1α in Colorectal Cancer Tissues[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(5): 354-358.

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结直肠癌组织中葡糖醛酸转移酶2B同工酶和肝细胞核因子的表达研究

Expression of UDP_glucuronosyl_transferase 2B and Hepatic Nuclear Factor 1α in Colorectal Cancer Tissues

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