关键词: 北豆根PE2成分, 肿瘤细胞, 细胞凋亡

Abstract: 北豆根提取成分PE2诱导胃癌细胞凋亡的实验研究 单保恩1/梁文杰1/刘东青2/任风芝3 (1.河北医科大学第四医院科研中心，河北　石家庄 050011；2.邯郸市第一医院；3.华北制药集团新药研究中心) () 　　【摘要】 　　【关键词】 北豆根PE2成分； 肿瘤细胞； 细胞凋亡 　　中图分类号： R730.32　　　　文献标识码： A　　　　文章编号： 1004－616X(2006)04－0269－04 　　【ABSTRACT】 BACKGROUND ＆ AIM: To study the effects of tumor cell apoptosis induced by Rhizoma Menipermi Extracts-PE2 and their mechanisms. MATERIAL AND METHODS: Rhizoma Menipermi extract with ethanol was preliminary purified using three steps method. PE2 was isolated and purified further by Pole Chromatogram. The apoptosis of tumor cells BGC823 induced by PE2 was studied by fluorescent staining, flow-cytometry (FCM), electron microscopy and DNA agarose gel electrophoresis technique. RESULTS: PE2 could induce apoptosis of BGC823 cells in a dose-and time-dependent relationship. After treatment with PE2, the BGC823 cells received some typical morphologic features and super-microstructural changes of apoptosis, including cell shrinkage chromatin concentrating on nuclear membrane or forming crescent, nuclear condensation, nuclear fragmentation and formation of apoptotic bodies. Some typical subdiploid peaks before G0/G1 phase were observed. DNA agarose gel electrophoresis showed characteristic "DNA ladder" pattern. After treatment with 5 μg/ml PE2, the cells in G0/G1 phase were blocked and increased markedly. But the cells in S phase were blocked after treatment with 50 μg/ml PE2. And the expression of bax was up-regulated. CONCLUSION: Rhizoma Menispermi Extract-PE2 induced tumor cell apoptosis, which was an important mechanism of its anti-tumor effect. Futher purification and study of their mechanisms can produce the scientific evidence for developing and manufacturing new antitumor drugs. 【KEY WORDS】 收稿日期： ； 修订日期： 基金项目： 国家自然基金，No.3037153；河北省自然基金，No.C2004000610 作者简介： 单保恩(1962－　)，男，河北省广平县人，教授，博士，博士生导师，研究方向：肿瘤免疫学。电话：0311(86033941－283)；传真：0311－86992004；Email:

Key words: Rhizoma Menipermi extracts PE2, tumor cell line, cellular apoptosis