关键词: bcl-2, 肝细胞癌, 基因转染, 基因表达

Abstract: Purpose and Methods : To establish a hepatocellular carcinoma (HCC) cell st rain that expresses bcl2-protein stably the ret rovirus expression vector pDOR2SB containing human bcl-2 cDNA was t ransduced into bcl-2 protein negative cell line HCC-9204 by lipid2mediated gene t ransfection method. The bcl-2 t ransfected cells were obtained after being selected with G-418 , and the expression of bcl-2 was detected by immunocytochemist ry after the cells being expanded. The t ransfected cells were cloned by a limited dilution method , and bcl-2 expression in the monoclonal cells was detected with flow cytometer. Results : Bcl-2 protein was expressed in most of the HCC-9204 cells t ransfected with pDOR2SB , but was negative in the HCC-9204 cells t ransfected with pDOR vector and nont ransfected HCC29204 cells. After being cloned for 3 times continually , the monoclonal cell strain that expressed bcl-2 protein at a 100 % positive rate was obtained. Conclusion : The HCC cell st rain that expressed bcl-2 protein stably , was established successfully by gene t ransfection method.