补充叶黄素及EGCG对人体抗氧化活性及DNA氧化损伤的影响

doi:10.3969/j.issn.1004-616x.2015.05.009

癌变·畸变·突变 ›› 2015, Vol. 27 ›› Issue (5): 370-374.doi: 10.3969/j.issn.1004-616x.2015.05.009

补充叶黄素及EGCG对人体抗氧化活性及DNA氧化损伤的影响

滕倩, 马婷婷, 孙永叶, 张华琦, 马爱国

青岛大学医学院医学营养研究所, 山东青岛 266021

收稿日期:2015-05-22 修回日期:2015-09-07 出版日期:2015-09-30 发布日期:2015-09-30
通讯作者: 马爱国,E-mail:magfood@126.com E-mail:magfood@126.com
作者简介:滕倩,E-mail:tengqianstu@126.com。
基金资助:
国家科技支撑项目(2012BAD33B01-2)

Effects of lutein and EGCG supplementation on antioxidative activity and DNA oxidative damage

TENG Qian, MA Tingting, SUN Yongye, ZHANG Huaqi, MA Aiguo

Institute of Human Nutrition, Medical College of Qingdao University, Qingdao 266021, Shandong, China

Received:2015-05-22 Revised:2015-09-07 Online:2015-09-30 Published:2015-09-30

摘要/Abstract

摘要：

目的:探讨补充叶黄素与表没食子儿茶素没食子酸酯(EGCG)对机体抗氧化活性及DNA氧化损伤的影响。方法:选择40名健康青年志愿者,随机分为4组,分别为叶黄素组(20 mg/d)、EGCG组(270 mg/d)、叶黄素(20 mg/d)+EGCG(270 mg/d)组和正常对照组。3个实验组志愿者连续补充叶黄素和/或EGCG 20 d,正常对照组未给任何补充。试验前及试验第21天分别留取受试者清晨空腹静脉血5 mL,分离血浆并收集淋巴细胞。高效液相色谱法检测血浆中叶黄素和EGCG浓度,测定总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量,采用碱性单细胞凝胶电泳法测定外周血淋巴细胞DNA损伤状况。结果:经过20 d后,叶黄素组和叶黄素+EGCG组受试者血浆中叶黄素水平较干预前分别升高了257%和175%(P<0.01)。与正常对照组相比,干预后叶黄素组受试者血浆中MDA含量、T-SOD活力明显下降、GSH-Px活力显著升高(P均<0.05),EGCG组受试者血浆中MDA含量、T-SOD、GSH-Px活力无明显差异(P均>0.05),叶黄素和EGCG对机体抗氧化活力无交互作用。DNA损伤分析显示,叶黄素组、EGCG组中由低、中浓度H₂O₂诱导的外周血淋巴细胞DNA损伤均显著低于对照组(P<0.05),而由高浓度 H₂O₂诱导的DNA氧化损伤及自发损伤与对照组比较差异无统计学意义,叶黄素和EGCG对DNA损伤的影响无交互作用(P均>0.05)。结论:叶黄素、EGCG单独补充均可降低一定浓度H₂O₂诱导的DNA氧化损伤,叶黄素单独补充也可增强机体抗氧化活性;叶黄素与EGCG对机体抗氧化活性及DNA氧化损伤的影响均无交互作用。

关键词: 叶黄素, 素没食子儿茶素没食子酸酯, 抗氧化, DNA损伤

Abstract:

OBJECTIVE: To investigate the effects of lutein and epigallocatechin gallate (EGCG) supplementation on the antioxidant activity and DNA damage. METHODS:A total of 40 healthy young adults were randomly divided into four groups (n=10 in each group), including lutein group (20 mg/d), EGCG group (270 mg/d), lutein (20 mg/d)+EGCG (270 mg/d) group and normal control group. Three experimental groups of volunteers were treated with lutein and or EGCG for 20 d while the control group wasn't given any supplement. 5 mL fasting blood was collected at the beginning and the end of the trial. Plasma lutein and EGCG concentrations were measured by HPLC detector systems. Plasma total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and malondial dehyde (MDA) levels were measured by kits. Peripheral blood lymphocyte DNA damage was analyzed by single-cell gel electrophoresis (SCGE). RESULTS:After supplementation for 20 days, plasma lutein concentration of the subjects in the lutein group and lutein+EGCG group increased by 257% and 175%(P<0.01). Plasma MDA and T-SOD concentrations of the subjects in the lutein group were significantly lower than those of the control group, while plasma GSH-Px concentration was significantly increased (P<0.05). No significant difference was found in the antioxidant activity of the subjects in the EGCG group. The combined supplementation of lutein and EGCG had no interaction on the antioxidant activity. DNA damage analysis showed that low- and medium- concentration H₂O₂-induced DNA damage were markedly lower in either lutein or EGCG group than those in the control group (P<0.05). However high- concentration H₂O₂-induced and spontaneous DNA damage in both groups did not show any significant difference with those in the control group. The combined supplementation of lutein and EGCG had no interaction on the DNA damage (P>0.05). CONCLUSION:Lutein or EGCG supplementation alone could effectively reduce DNA damage. Lutein supplementation enhanced antioxidant activity, but EGCG did not. Lutein and EGCG did not show any interaction on antioxidant activity and DNA damage.

Key words: lutein, epigallocatechin gallate, antioxidation, DNA damage

中图分类号:

R151.3

滕倩, 马婷婷, 孙永叶, 张华琦, 马爱国. 补充叶黄素及EGCG对人体抗氧化活性及DNA氧化损伤的影响[J]. 癌变·畸变·突变, 2015, 27(5): 370-374.

TENG Qian, MA Tingting, SUN Yongye, ZHANG Huaqi, MA Aiguo. Effects of lutein and EGCG supplementation on antioxidative activity and DNA oxidative damage[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2015, 27(5): 370-374.

参考文献

[1] Benzie IF. Evolution of antioxidant defence mechanisms [J]. Eur J Nutr,2000,39(2):53-61.

[2] Liu S,Lee IM,Ajani U,et al. Intake of vegetables rich in carotenoids and risk of coronary heart disease in men:The physicians' health study [J]. Int J Epidemiol,2001,30(1): 130-135.

[3] Tanaka T,Shnimizu M,Moriwaki H. Cancer chemoprevention by carotenoids [J]. Molecules,2012,17(3):3202-3242.

[4] Zhang L,Wei Y,Zhang J. Novel mechanisms of anticancer activities of green tea component epigallocatechin-3-gallate [J]. Anticancer Agents Med Chem,2014,14(6):779-786.

[5] Krinsky NI,Deneke SM. Interaction of oxygen and oxy-radicals with carotenoids [J]. J Natl Cancer Inst,1982,69(1):205-210.

[6] 马爱国,郑樱. 叶黄素 [J]. 营养学报,2013,35(6):531-534.

[7] 陈丹丹. 茶多酚抗氧化作用的应用研究进展 [J]. 中药与临床,2014,5(3):59-64.

[8] Lu CC,Yen GC. Antioxidative and anti-inflammatory activity of functional foods [J]. Curr Opin Food Sci,2015,2: 1-8.

[9] Bertram B,Bollow U,Rajaee-Behbahani N,et al. Induction of poly(ADP-ribosyl)ation and DNA damage in human peripheral lymphocytes after treatment with (-)-epigallocatechin-gallate [J]. Mutat Res,2003,534(1/2):77-84.

[10] Santocono M,Zurria M,Berrettini M,et al. Influence of astaxanthin,zeaxanthin and lutein on DNA damage and repair in UVA-irradiated cells [J]. J Photochem Photobiol B,2006, 85(3):205-215.

[11] 赵彦会,张辉珍,梁惠,等. 血浆样品中叶黄素的快速测定 [J]. 卫生研究,2015,44(1):124-131.

[12] Lee MJ,Wang ZY,Li H,et al. Analysis of plasma and urinary tea polyphenols in human subjects [J]. Cancer Epidemiol Biomarkers Prev,1995,4(4):393-399.

[13] 马爱国,韩秀霞,刘四朝. 两种DNA断裂损伤检测方法敏感性的比较 [J]. 遗传,1997,19(1):36-38.

[14] Duthie SJ,Ma A,Ross MA,et al. Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes [J]. Cancer Res,1996,56(6):1291-1295.

[15] Zhao X,Aldini G,Johnson EJ,et al. Modification of lymphocyte DNA damage by carotenoid supplementation in postmenopausal women [J]. Am J Clin Nutr,2006,83(1): 163-169.

[16] Herrero-Barbudo C,Soldevilla B,Perez-Sacristan B,et al. Modulation of DNA-induced damage and repair capacity in humans after dietary intervention with lutein-enriched fermented milk [J]. PLoS One,2013,8(9):e74135.

[17] Chow HH,Cai Y,Hakim IA,et al. Pharmacokinetics and safety of green tea polyphenols after multiple-dose administration of epigallocatechin gallate and polyphenon E in healthy individuals [J]. Clin Cancer Res,2003,9(9):3312-3319.

[18] Pietta P,Simonetti P,Gardana C,et al. Relationship between rate and extent of catechin absorption and plasma antioxidant status [J]. Biochem Mol Biol Int,1998,46(5):895-903.

[19] Kimura M,Umegaki K,Kasuya Y,et al. The relation between single/double or repeated tea catechin ingestions and plasma antioxidant activity in humans [J]. Eur J Clin Nutr,2002, 56(12):1186-1193.

[20] Li L,Chen CY,Aldini G,et al. Supplementation with lutein or lutein plus green tea extracts does not change oxidative stress in adequately nourished older adults [J]. J Nutr Biochem, 2010,21(6):544-549.

补充叶黄素及EGCG对人体抗氧化活性及DNA氧化损伤的影响

Effects of lutein and EGCG supplementation on antioxidative activity and DNA oxidative damage

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