贲门腺癌中Sox17蛋白表达及其与β-catenin、cyclin D1蛋白相关性研究

doi:10.3969/j.issn.1004-616x.2017.02.006

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (2): 106-110,120.doi: 10.3969/j.issn.1004-616x.2017.02.006

贲门腺癌中Sox17蛋白表达及其与β-catenin、cyclin D1蛋白相关性研究

潘盼¹, 邝钢², 郭艳丽², 郭炜², 董稚明², 杨飞¹

1. 山东大学附属济南市中心医院病理科, 山东济南 250013;
2. 河北医科大学第四医院肿瘤研究所, 河北石家庄 050011

收稿日期:2016-03-11 修回日期:2017-02-10 出版日期:2017-03-31 发布日期:2017-03-31
通讯作者: 杨飞,E-mail:yangf-2008@163.com E-mail:yangf-2008@163.com
作者简介:潘盼,E-mail:ppaann1120@163.com。
基金资助:
河北省自然科学基金（H2013206315）；河北省医学科学研究重点课题计划项目（20130543）

Expression of Sox17 protein and correlation with β-catenin and cyclin D1 protein in gastric cardia adenocarcinoma

PAN Pan¹, KUANG Gang², GUO Yanli², GUO Wei², DONG Zhiming², YANG Fei¹

1. Department of Pathology, Jinan Central Hospital of Shandong University, Jinan 250013, Shandong;
2. Cancer Institute, Fouth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China

Received:2016-03-11 Revised:2017-02-10 Online:2017-03-31 Published:2017-03-31

摘要/Abstract

摘要：

目的：检测贲门腺癌中Sox17蛋白表达及其与β-catenin和cyclin D1蛋白的相关性，探讨三者的表达状态与贲门腺癌发生发展的关系。方法：应用免疫组织化学法检测78例贲门腺癌及相应癌旁非肿瘤组织中Sox17、β-catenin及cyclin D1蛋白的表达情况及其与临床病理指标的关系。结果：在贲门腺癌组织中，Sox17、β-catenin和cyclin D1的阳性表达率分别为47.4% （37/78）、83.3% （65/78）和70.5%（55/78）；在癌旁非肿瘤组织中，三者的阳性表达率分别为69.2%（54/78）、62.8%（49/78）和44.9% （35/78），差异均有统计学意义（P < 0.01）。Sox17蛋白的阳性表达率与β-catenin和cyclin D1的阳性表达率均呈负相关（r=-0.264，P < 0.05；r=-0.287，P < 0.05）；β-catenin与cyclin D1的阳性表达率呈显著正相关（r=0.913，P < 0.05）。Sox17在I、II期的阳性表达率明显高于III、IV期，二者比较差异有统计学意义（P < 0.05）。β-catenin、cyclin D1的阳性表达率与淋巴结转移状态有关（P < 0.05）。结论：Sox17蛋白在贲门腺癌中低表达，其可能通过异常活化Wnt/β-catenin信号转导通路，上调β-catenin蛋白及其下游靶蛋白cyclin D1的表达水平参与了贲门腺癌的发生发展。

关键词: Sox17, β-catenin, cyclin D1, Wnt信号转导通路, 贲门腺癌

Abstract:

OBJECTIVE: To investigate expression of Sox17,β-catenin and cyclin D1 proteins in gastric cardia adenocarcinoma(GCA),and to establish their relationship with pathogenesis of GCA. METHODS: Immunohistochemistry method was applied to examine the expression of Sox17,β-catenin and cyclin D1 proteins in 78 samples of GCA and the corresponding adjacent non-cancerous tissues．RESULTS: In GCA tissues,the frequency of Sox17,β-catenin and cyclin D1 expression was 47.4% (37/78),83.3% (65/78) and 70.5%(55/78),respectively,which was significantly lower than that in corresponding adjacent non-cancerous tissues:69.2%(54/78),62.8%(49/78) and 44.9%(35/78),(P < 0.01). The frequency of β-catenin ectopic expression was 83.3%(65/78) in GCA tissues which was significantly higher than that in corresponding adjacent non-cancerous tissues of 62.8%(49/78) (P < 0.01). Sox17 expression was negatively correlated with ectopic expression of β-catenin and cyclin D1 (r=-0.264,P < 0.05;r=-0.287,P < 0.05). Sox17 were significantly correlated with tumor stage (P=0.045). β-catenin and cyclin D1 were significantly correlated with lymph node metastasis(P < 0.05). CONCLUSION: Low level expression of Sox17 protein may play an important role in the development of GCA via aberrantly activating the canonical Wnt/ β-catenin signaling pathway through influencing the expression of β-catenin and cyclin D1.

Key words: Sox17, β-catenin, cyclin D1, Wnt signal pathway, gastric cardia adenocarcinoma

中图分类号:

R735.2

潘盼, 邝钢, 郭艳丽, 郭炜, 董稚明, 杨飞. 贲门腺癌中Sox17蛋白表达及其与β-catenin、cyclin D1蛋白相关性研究[J]. 癌变·畸变·突变, 2017, 29(2): 106-110,120.

PAN Pan, KUANG Gang, GUO Yanli, GUO Wei, DONG Zhiming, YANG Fei. Expression of Sox17 protein and correlation with β-catenin and cyclin D1 protein in gastric cardia adenocarcinoma[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 106-110,120.

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贲门腺癌中Sox17蛋白表达及其与β-catenin、cyclin D1蛋白相关性研究

Expression of Sox17 protein and correlation with β-catenin and cyclin D1 protein in gastric cardia adenocarcinoma

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