锶在兔体内部分组织器官分布与富集规律的模拟研究

doi:10.3969/j.issn.1004-616x.2017.02.007

癌变·畸变·突变 ›› 2017, Vol. 29 ›› Issue (2): 111-114.doi: 10.3969/j.issn.1004-616x.2017.02.007

锶在兔体内部分组织器官分布与富集规律的模拟研究

何映雪, 闫冬, 丁库克, 姜晓燕

中国疾病预防控制中心辐射防护与核安全医学所放射生态研究室, 北京 100088

收稿日期:2017-01-16 修回日期:2017-02-28 出版日期:2017-03-31 发布日期:2017-03-31
通讯作者: 姜晓燕,E-mail:fangsheshengtai@126.com E-mail:fangsheshengtai@126.com
作者简介:何映雪,E-mail:yingxuehe@sina.com。
基金资助:
国家自然科学基金面上项目（41672228）

Simulation study on the distribution and accumulation of strontium in some organs of rabbits

HE Yingxue, YAN Dong, DING Kuke, JIANG Xiaoyan

Department of Radioecology, National Institute for Radiological Protection, Chinese Centre for Disease Control and Prevention, Beijing 100088, China

Received:2017-01-16 Revised:2017-02-28 Online:2017-03-31 Published:2017-03-31

摘要/Abstract

摘要：

目的：研究锶在兔体内不同器官中的分布和富集规律。方法：将日本大耳白兔分6组，1个空白对照组和5个实验组，每组4个平行样。空白组（第1组）白兔喂去离子水，5个实验组（第2~6组）白兔饮用添加了六水氯化锶（浓度0.002 g/mL）的去离子水，隔天灌喂1次，第2~6组饮用水体积依次递增分别为1、2、3、4、5 mL。30 d后取兔血液、肝脏和后肢胫骨，样品经烘干、粉碎、硝解后送检。利用电感耦合等离子体发射光谱仪（ICP-AES）检测样品的锶含量。结果：白兔血液锶含量随着摄入锶总量的增加而增加，呈正相关关系，线性拟合R2值为0.82。肝脏中的锶含量与摄入锶总量之间无明显相关关系。后肢胫骨中锶含量随着摄入锶总量的增加而增加，呈正相关关系，线性拟合R²值为0.98。血液和胫骨中的锶含量呈正相关关系，线性拟合R²值为0.76。结论：锶进入生物体后经过血液循环最后富集在骨骼中。

关键词: 锶, 骨骼, 血液, 日本大耳白兔, 富集

Abstract:

OBJECTIVE: We used the Japanese big-ear rabbits to study the distribution and accumulation of strontium in their different organs. METHODS: 24 Japanese big-ear rabbits were randomly allocated into one blank group and five experimental groups. Rabbits of the blank group (group 1) were fed with deionized water while the experimental group were fed with deionized water plus the addition of strontium chloride hexahydrate every other day. The concentration of strontium chloride was 0.002 g/mL. The volume of water with strontium that was given to the rabbits from groups 2 to 6 were 1,2,3,4 and 5 mL respectively. After 30 days of oral treatment,samples of blood,liver and back leg tibialis were taken. All samples were dried,mashed and decompounded by nitric acid. The concentration of strontium was analyzed by inductively coupled plasma atomic emission spectrometry (ICP-AES). RESULTS: Plasma strontium concentrations in rabbits positively related to oral treatment dose with strontium chloride. R2 of linear fitting method was 0.82. Liver strontium concentrations in rabbits showed no significant correlation with oral treatment dose. Back leg tibialis strontium concentrations in rabbits positively related to oral treatment dose with strontium chloride. R2 of linear fitting method was 0.98. Plasma strontium concentrations positively related to back leg tibialis strontium concentrations. R2 of linear fitting method was 0.76. CONCLUSION: Strontium was absorbed by the body from the gut,transferred through plasma and concentrated in bone.

Key words: strontium, bone, plasma, Japanese big-ear rabbit, concentration

中图分类号:

R811.5

何映雪, 闫冬, 丁库克, 姜晓燕. 锶在兔体内部分组织器官分布与富集规律的模拟研究[J]. 癌变·畸变·突变, 2017, 29(2): 111-114.

HE Yingxue, YAN Dong, DING Kuke, JIANG Xiaoyan. Simulation study on the distribution and accumulation of strontium in some organs of rabbits[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(2): 111-114.

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锶在兔体内部分组织器官分布与富集规律的模拟研究

Simulation study on the distribution and accumulation of strontium in some organs of rabbits

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