关键词: 烟花苷, 半乳糖胺, 肝损伤, 保肝作用

Abstract: OBJECTIVE: To study protective effect of nicotiflorin from Nymphaea candida on D-Gal-induced liver injury in mice. METHODS: Sixty healthy adult mice were randomly divided into six groups:control,model,dimethyl diphenyl bicarboxylate (DDB,150 mg/kg) and nicotiflorin (25,50,100 mg/kg) groups. Chemicals were intragastrically administrated for seven days. One hour after the last administration,mice in all except the control group were intraperitoneally injection with D-Gal (800 mg/kg,0.02 mL/g). All mice were sacrificed at the 8th hour,blood was collected from the eyeball,and serum ALT,AST,IL-1β,IL-6,TNF-α and INF-γ were determined. Mice were killed cervical dislocation,the quality of the body after the autopsy,the liver,spleen,the quality of the organ coefficient,the liver homogenate were placed in -20 freezer. The left hepatic lobes at 0.5 cm from the edge of tissues were fixed with 10% formalin,dehydrated,transparentized,paraffined and embedding slices were observes under light microscope for pathological changes. RESULTS: Compared with the model group,nicotiflorin significantly reduced serum ALT and AST activity of rats with acute liver injury (P < 0.01) at doses greater than 50 mg/kg, significantly improved liver homogenate of SOD and GSH activity at higher than 50 mg/kg (P < 0.05),significantly decreased MDA and NO content in the liver (P < 0.05),IL-1,IL-6,reduced inflammation and promoted the level of INF-γ and TNF-α in serum of mice (P < 0.05),reduced liver and spleen index (P < 0.05) at a dose of more than 50 mg/kg. Liver pathological examinations showed that,compared with the model group,nicotiflorin significantly reduced the degree of liver cell injury. Inflammatory cell infiltration was significantly decreased,including high,medium and low dose group compared to the extent of the damage and repair. CONCLUSION: The protective effect of D-Gal on acute liver injury in mice was related to the promotion of antioxidant enzymes and the expression of proinflammatory cytokines.

Key words: Nymphaea candida presl, nicotiflorin, liver injury, hepatoprotective