筛选化学过敏原的直接肽反应试验的建立

doi:10.3969/j.issn.1004-616x.2018.02.014

癌变·畸变·突变 ›› 2018, Vol. 30 ›› Issue (2): 150-154.doi: 10.3969/j.issn.1004-616x.2018.02.014

筛选化学过敏原的直接肽反应试验的建立

胡培丽, 刘师卜, 张会亮, 李波, 王钢力

中国食品药品检定研究院, 北京 100050

收稿日期:2017-10-24 修回日期:2018-03-14 出版日期:2018-03-30 发布日期:2018-03-30
通讯作者: 王钢力,E-mail:wanggl@nifdc.org.cn E-mail:wanggl@nifdc.org.cn
作者简介:胡培丽,E-mail:hupeili-1981@163.com
基金资助:
中青年基金项目（2013NC3）

Development of a peptide reactivity assay for screening chemical allergens'

HU Peili, LIU Shibo, ZHANG Huiliang, LI Bo, WANG Gangli

National Institutes for Food and Drug Control, Beijing 100050, China

Received:2017-10-24 Revised:2018-03-14 Online:2018-03-30 Published:2018-03-30

摘要/Abstract

摘要： 目的：建立筛选化学过敏原的体外替代法——直接肽反应试验。方法：赖氨酸/半胱氨酸肽与受试化学物反应24 h，经高效液相色谱检测肽损耗评价受试化学物的致敏性及其肽反应活性。结果：12种不同致敏级别化学物肽损耗率均值大于6.38%，为致敏阳性，反应活性不同；6种非致敏物肽损耗率均值小于6.38%，为致敏阴性，与LLNA致敏分类一致；致敏性未知的氯仿和苯肽损耗率均小于6.38%，为致敏阴性。结论：直接肽反应试验可较好地评价化学物的皮肤致敏性及致敏能力，该法简单、快速，适宜高通量筛选化学物致敏性，可推广应用于化学物安全性评价。

关键词: 肽反应, 过敏原, 皮肤致敏性, 高效液相色谱

Abstract: OBJECTIVE: To develop an in vitro alternative approach to the direct peptide reactivity assay (DPRA) for screening chemical allergens. METHODS: Lysine/cysteine peptides were incubated with test chemicals for 24 h and high performance liquid chromatography was performed. Depletion of the peptide was used as an indication of skin sensitization potential of chemicals. RESULTS: 12 chemicals representing allergens of different potencies caused depletion of greater than 6.38% with different reactivity,6 nonsensitizers gave depletion of less than 6.38%,which was consistent with LLNA sensitization classification. Unknown allergenic chloroform and benzene both yielded less than 6.38% depletion,which were judged as negative. CONCLUSION: The direct peptide reactivity assay appears to be a reliable predictor of sensitization potential for chemicals. In addition,it is simple,rapid and suitable for high throughput screening of chemical sensitization. Therefore,the assay can possibly be used for safety evaluation of chemicals.

Key words: peptide reactivity, allergens, skin sensitization, high performance liquid chromatograph

中图分类号:

R758.22

胡培丽, 刘师卜, 张会亮, 李波, 王钢力. 筛选化学过敏原的直接肽反应试验的建立[J]. 癌变·畸变·突变, 2018, 30(2): 150-154.

HU Peili, LIU Shibo, ZHANG Huiliang, LI Bo, WANG Gangli. Development of a peptide reactivity assay for screening chemical allergens'[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(2): 150-154.

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筛选化学过敏原的直接肽反应试验的建立

Development of a peptide reactivity assay for screening chemical allergens'

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