癌变·畸变·突变 ›› 2020, Vol. 32 ›› Issue (2): 98-104.doi: 10.3969/j.issn.1004-616x.2020.02.004

• 论著 • 上一篇    下一篇

乙酰肝素酶、E-cadherin、N-cadherin和vimentin蛋白在胃癌中的表达及其意义

申志华1, 黄萨础拉1, 马秀梅2   

  1. 1. 内蒙古医科大学, 内蒙古 呼和浩特 010000;
    2. 内蒙古医科大学附属医院病理科, 内蒙古 呼和浩特 010000
  • 收稿日期:2019-05-27 修回日期:2019-11-25 出版日期:2020-03-30 发布日期:2020-04-10
  • 通讯作者: 马秀梅,E-mail:doctor-maxiumei0471@126.com E-mail:doctor-maxiumei0471@126.com
  • 作者简介:志华,E-mail:shenzhihuaya@126.com;黄萨础拉,E-mail:1005381124@qq.com。
  • 基金资助:
    国家自然科学基金(81341132);中支地学校匹配经费

Expression of heparanase, E-cadherin, N-cadherin and vimentin in gastric cancers

SHEN Zhihua1, HUANG Sachula1, MA Xiumei2   

  1. 1. Inner Mongolia Medical University, Hohhot 010000;
    2. Department of Pathology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia, China
  • Received:2019-05-27 Revised:2019-11-25 Online:2020-03-30 Published:2020-04-10

摘要: 目的: 探讨人胃癌组织中乙酰肝素酶(HPA)、上皮标志物E-cadherin、间质标志物N-cadherin和vimentin蛋白的表达及其与人胃癌临床病理指标的关系,以及HPA与E-cadherin、N-cadherin、vimentin蛋白之间的关系。方法: 应用免疫组织化学方法检测91例人胃癌组织中HPA、E-cadherin、N-cadherin和vimentin蛋白表达情况;采用卡方检验,检测这几种蛋白阳性表达率与人胃癌不同病理指标的关系;同时采用Spearman等级相关分析HPA与E-cadherin、N-cadherin、vimentin蛋白之间的关系。结果: 人胃癌组织中HPA、E-cadherin、N-cadherin和vimentin蛋白阳性表达率为75.82%、51.65%、54.95%和23.08%。91例人胃癌组织中HPA、E-cadherin、N-cadherin和vimentin蛋白的阳性表达率与胃癌患者年龄、性别、胃癌大小均无明显相关(P > 0.05),而与人胃癌发生部位、分化程度和有无淋巴结转移、侵袭程度有关(P < 0.05)。HPA、N-cadherin和vimentin蛋白在贲门部阳性表达率明显高于胃体、幽门及胃窦胃癌;低分化者、有淋巴结转移者和胃癌侵袭深处这3种蛋白阳性表达率明显高于高分化者、无淋巴结转移者和胃癌起始部位;E-cadherin蛋白在幽门及胃窦部胃癌阳性表达率明显高于贲门和胃体胃癌;高中分化者、无淋巴结转移者明显高于低分化者和有淋巴结转移者。E-cadherin蛋白阳性表达率在侵袭深处明显低于起始部位。经Spearman等级相关分析显示,人胃癌组织中HPA与E-cadherin蛋白的阳性表达率呈负相关(r=-0.341,P < 0.05);而与N-cadherin(r=0.366,P < 0.05)和vimentin(r=0.284,P < 0.05)蛋白阳性表达率呈正相关。结论: 人胃癌组织中HPA、N-cadherin和vimentin蛋白在低分化者、有淋巴结转移者和胃癌侵袭深处高表达;E-cadherin蛋白在高中分化胃癌,无淋巴结转移者和起始部位高表达。HPA与N-cadherin、vimentin蛋白阳性表达率呈正相关,HPA与E-cadherin蛋白的阳性表达率呈负相关,可见HPA蛋白与间质标志物N-cadherin和vimentin蛋白表达增强有关,而与上皮标志物E-cadherin蛋白缺失或减少有关,因此HPA可能诱导人胃癌组织发生上皮间质转化。

关键词: 乙酰肝素酶, 胃癌, 免疫组化, 上皮间质转化

Abstract: OBJECTIVE: To investigate expression of heparanase (HPA),epithelial marker E-cadherin, interstitial marker N-cadherin and vimentin protein in human gastric cancers and their relationship with clinical-pathological indicators of human gastric cancer. METHODS: Expressions of HPA, E-cadherin, N-cadherin and vimentin proteins in 91 cases of human gastric cancers were detected by immunohistochemical methods. The relationships between the positive expression rates of these proteins and different pathological indicators of human gastric cancer were detected using chi-square test. Furthermore,the relationships between HPA and E-cadherin,HPA and N-cadherin,vimentin protein were analyzed using Spearman rank correlation. RESULTS: The positive expression rates of HPA,E-cadherin,N-cadherin and vimentin proteins in human gastric cancer tissues were 75.82%, 51.65%, 54.95% and 23.08%, respectively. Positive expression rates of HPA, E-cadherin,N-cadherin and vimentin proteins in 91 cases of human gastric cancer tissues related to the age,sex,and size of gastric cancer patients (P > 0.05),but were related to the location and degree of differentiation of human gastric cancer. It was related to the degree of lymph node metastasis and invasion (P < 0.05). Positive expression rates of HPA,N-cadherin and vimentin proteins in the gastric cardia were significantly higher than those in the gastric body,pyloric and antral gastric cancers. Poorly differentiated,lymph node metastatic and invasive gastric cancer were significantly higher than those in well differentiated,non-lymph node metastatic and initial gastric cancer. Positive expression rates of E-cadherin protein in pyloric and antral gastric cancer were significantly higher than those in cardiac and gastric body gastric cancers. Patients with high school differentiation and without lymph node metastasis were significantly higher than those with low differentiation and lymph node metastasis. Positive expression rates of E-cadherin protein in the depth of invasion were significantly lower than that in the initial site. The Spearman grade correlation analyses show that the positive expression rates of HPA were negatively correlated with that of E-cadherin protein in human gastric cancer tissues (r=0.341,P < 0.05),but positively correlated with the positive expression rates of N-cadherin (r=0.366, P < 0.05) and vimentin (r=0.284,P < 0.05). CONCLUSION: In human gastric cancers,the positive expression rates of HPA,N-cadherin and vimentin proteins were high in poorly differentiated,lymph node metastasis and invasive depth of gastric cancer,while the positive expressions of E-cadherin proteins were high in moderately differentiated gastric cancers, non-lymph node metastasis and initial sites. There was a positive correlation between HPA and N-cadherin proteins,and a positive correlation between HPA and vimentin proteins,and a negative correlation between HPA and E-cadherin protein expression. It can be seen that HPA protein was related to the increased expression of stromal marker N-cadherin and vimentin protein, and to the loss or decrease of epithelial marker E-cadherin protein, therefore HPA may induce epithelial-mesenchymal transformation in human gastric cancer tissue.

Key words: heparanase, gastric cancer, immunohistochemical staining, epithelial-mesenchymal transformation

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