癌变·畸变·突变 ›› 2002, Vol. 14 ›› Issue (4): 214-217.doi: 10.3969/j.issn.1004-616x.2002.04.003

• 论著 • 上一篇    下一篇

饮水微囊藻毒素在大鼠肝癌发生期间对细胞增殖与凋亡的影响

陈　华, 孙昌盛, 胡志坚, 汪家梨, 王章敬   

  1. 福建医科大学公共卫生学院　职业与环境卫生学系, 福建　福州　350004
  • 收稿日期:2002-04-04 修回日期:2002-07-12 出版日期:2002-10-30 发布日期:2002-10-30
  • 通讯作者: 陈　华

EFFECTS OF MICROCYSTIC TOXINS IN DRINKING WATER ON CELL PROLIFERATION AND APOPTOSIS DURING THE COURSE OF HEPATOCARCINOGENESIS

CHEN Hua, SUN Chang-sheng, HU Zhi-jian, etal.   

  1. School of Public Htealth, Fujian Medical University, Fuzhou 350004, China
  • Received:2002-04-04 Revised:2002-07-12 Online:2002-10-30 Published:2002-10-30
  • Contact: CHEN Hua

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摘要： 目的: 研究经饮水摄入微囊藻毒素(MC) 在大鼠肝癌发生期间对细胞增殖与凋亡的影响,进而探讨微囊藻毒素促肝癌机制。方法:50 只Wistar 大鼠随机分为4 组,A 组(空白对照) :B 组(DEN 启动对照) ;C 组(DEN 处理,饮含MC 藻水) :D 组(DEN理,腹腔注射MCLR 纯毒素) 、应用LSAB 免疫组化技术检测大鼠肝癌前病变病灶中PCNA、Bcl22 和Bax 蛋白的表达。结果:各处理组PCNA 平均积分光密度值(AIOD) 均较A 组0. 0013 明显升高,分别为B 组0. 0033 ,C 组0. 0036 和D 组0. 0088 ,其中D 组与B 组比较有明显升高( P < 0. 05) 。饮水摄入或腹腔注射微囊藻毒素均可使Bcl22 表达上调,Bax 表达下调。结论:促进调控细胞增殖与凋亡的基因平衡失调. 使细胞失控性生长,可能是微囊藻毒素促肝癌作用的分子机制之一。

关键词: 微囊藻毒素, 基因表达, 肝肿瘤, 饮水

Abstract: Purpose : To study the effect s of drinking water contaminated with microcystins (MC) on cell proliferation and apoptosis during the course of hepatocarcinogenesis ,and to probe the mechanism of promoting hepatocarcinogenesis of microcystic toxins. Methods : 50 healthy Wistar rat s were randomly divided into 4 groups :group A (normal cont rol) ; group B (t reated with DEN only) ; group C (t reated with DEN and MC contaminated water) ; group D (t reated with DEN and microcystins i. p ) . The PCNA , Bcl22 and Bax protein expression in preneoplastic liver tissue were detected using immunohistochemical assays. Results : The average integrated optical densities (AIOD) of PCNA in the experimental groups were 0. 0033 (group B) , 0. 0036 ( group C ) and 0. 0088 (group D) respectively , and they were all significantly increased as compared to group A (0. 0013) . But as comparing to group B , only group D had significantly higher PCNA expression. Up2regulation of Bcl22 and down-regulation of Bax expression were found in group C and group D. Conclusion : Uncont rolled cell proliferation caused by unbalanced expression of Bcl-2 and Bax gene may be one of the mechanisms of hepatocarcinoma promoted by microcystins.

Key words: Microcystin toxins, gene expression, liver neoplasms, drinking water

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