癌变·畸变·突变 ›› 2002, Vol. 14 ›› Issue (4): 218-221.doi: 10.3969/j.issn.1004-616x.2002.04.004

• 论著 • 上一篇    下一篇

三氧化二砷诱导恶性黑色素瘤A375和B16细胞凋亡的研究

陈治文1, 夏　俊1, 胡守芬1, 马栋柱2, 王　丽1   

  1. 1. 蚌埠医学院生物化学与分子生物学教研室, 安徽　蚌埠　233003; 2. 复旦大学医学部生物化学与分子生物学教研室, 上海　200032
  • 收稿日期:2002-05-10 修回日期:2002-07-10 出版日期:2002-10-30 发布日期:2002-10-30
  • 通讯作者: 陈治文

THE STUDIES 0F APOPTOSIS 0N MELANOMA A375 AND B16 CELLS INDUCED BY ARSENIC TRIOXIDE

CHEN Zhi-wen1, XIA jun1, HU Shou-fen1, MA Dong-zhu2, WANG li1   

  1. 1. Departments of Biochemistry and Molecular Biology. Bengbu Medical College, Bengbu 233003, China; 2. Departments of Biochemistry and Molecular Biology, Fu Dan University of Medical School, Shanghai 200032, China
  • Received:2002-05-10 Revised:2002-07-10 Online:2002-10-30 Published:2002-10-30

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摘要： 目的: 研究不同浓度的三氧化二砷(As2O3) 对恶性黑色素瘤人A375细胞和小鼠B16细胞的凋亡诱导作用,为As2O3 治疗恶性黑色素瘤提供新的理论和实验依据。方法:用流式细胞术(FCM) 检测A375细胞和B16细胞的凋亡诱导及杀伤作用:用不同剂量的As2O3 (0. 1 mmol·L - 1 、0. 25 mmol·L - 1和0. 5 mmol/ L - 1) ,对小鼠进行腹腔注射(10 ml·kg - 1 ,连续注射10 d 后,检测小鼠血清与心、肝、肾有关的生化指标。结果:As2O3 浓度分别为5μmol·L - 1 、10μmol·L - 1和20μmol·L - 1时,A375和B16细胞的凋亡率分别为11. 85 %、55. 51 %、54. 90 %和9. 81 %、26. 45 %、9. 93 %:As2O3 诱导A375和B16细胞总的凋亡和坏死率分别为11. 90 %、55. 71 %、57. 40 %和12. 96 %、26. 99 %、87. 13 %;不同剂量的As2O3 (0. 1 mmol·L - 1～0. 5 mmol·L - 1 ) 对小鼠肝、肾和心肌等功能无明显影响,与对照组各项指标无显著性差异( P > 0. 05) 。结论:不同浓度的As2O3 能明显诱导恶性黑色素瘤A375及B16细胞凋亡和坏死,对A375细胞的凋亡诱导作用强于B16细胞。As2O3 (0. 1 mmol·L - 1～0. 5 mmol·L - 1) 对小鼠肝、肾和心肌等功能无明显损伤。

关键词: 三氧化二砷, 恶性黑色素瘤, 凋亡, A375细胞, B16细胞

Abstract: Purpose : To study the effect s of arsenic t rioxide (As2O3) on the apoptosis in melanoma A375 and B16 cells. Methods : Apoptosis and necrosis in A375 and B16 cells were analysed by flow cytomet ry ; The serum biochemical parameters in mice concerned with the functions of heart , liver and kidney were also measured after the mice were t reated with As2O3 . Results : As2O3 could induce apoptosis and necrosis of A375 and B16 cells. The rates of apoptosis in A375 and B16 cells were 11. 85 % , 55. 51 % , 54. 90 % , and 9. 81 % , 26. 45 % , 9. 93 % ,respectively , as t reated with different doses of As2O3 (5μmol·L - 1 , 10μmol·L - 1 and 20μmol·L - 1) ,respectively. The total rates of apoptosis and necrosis were 11. 90 % , 55. 71 % , 57. 40 % and 12. 96 % , 26. 99 % ,87. 13 %, respectively. No significant differences were found in serum biochemical parameters in mice t reated with As2O3 . Conclusion : As2O3 can induce apoptosis and necrosis of A375 and B16 cells , but does not cause damage to the functions of liver , kidney and heart in mice.

Key words: arsenic trioxide, melanoma, apoptosis, A375 cell, B16 cells

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