癌变·畸变·突变 ›› 1999, Vol. 11 ›› Issue (5): 236-238.doi: 10.3969/j.issn.1004-616x.1999.05.009

• 短篇报道 • 上一篇    下一篇

螺旋藻制剂对吐温20引发蚕豆根尖细胞微核发生率的抑制作用

梁 红 林炳水   

  1. 天津医科大学实验肿瘤研究室 天津 300070
  • 收稿日期:1999-03-11 修回日期:1999-06-15 出版日期:1999-09-30 发布日期:1999-09-30

RESTRAIN OF MICRONUCLEUS EFFECTS OF VICIA FABA ROOT TIP CELLS ON TOWIN20 BY EXTRACTS OF SPIRULINA PLATENSIS

Liang Hong  Lin Bingshui   

  1. Department of Ex perimental Oncology , Tianjin Medical University , Tianjin  300070
  • Received:1999-03-11 Revised:1999-06-15 Online:1999-09-30 Published:1999-09-30

摘要: 本文采用不同浓度的螺旋藻酒精提取液处理蚕豆根尖,随后以吐温20染毒,以观察螺旋藻制剂对吐温20引发的蚕豆根尖细胞微核发生率的抑制作用。从蚕豆根尖细胞微核发生率角度,经统计分析:以吐温20染毒的阳性对照组与阴性对照组比较P < 0. 01 ;经最佳浓度螺旋藻酒精提取液处理后以吐温20染毒组与阳性对照组比较P < 0. 01。结果表明:吐温20是引发显著微核效应的强诱变剂,而螺旋藻制剂对其引发的蚕豆根尖细胞微核发生率有明显抑制作用,进而从细胞水平探讨了螺旋藻在肿瘤发生发展的启动阶段的防癌作用机理。同时,本实验初步获得较为简易的螺旋藻有效成份提取方法。

关键词: 螺旋藻, 吐温20, 蚕豆根尖细胞微核

Abstract: In this paper , vicia faba roof tip cells are attended by ext ract s of spirulina platensis in different concent ration alcohol , following , poison t readed by towin20 , to observe the rest rain of micronucleus effect s of vicia faba roof tip cells on towin20 , by ext ract s of spirulina platensis. From the angle of micronucleus effect s of vicia faba roof tip cell , by statistical analysis : The positive cont rol of poison t readed by towin20 compared with the negative cont rol P < 0. 01 ; Poison t readed by towin20 after attended by ext ract s of spirulina platensis in best concent ration alcohol compared with the positive cont rol P < 0. 01. Result showed that towin20 is a st rong mutagen to cause significant micronucleus effect s. But , the ext ract s of spirulina platensis has significant rest rain to micronucleus effect s of vicia faba root tip cell on towin20 . Further more , approach the function of spirulina platensis to prevent cancer on the original of oncology on cell level. In the mean time , this experiment has tentatively gained a simpler and easier method to ext ract the effective composition of spirulina platensis.

Key words: spirulina platensis, towin20, micronucleus of vicia faba root tip cell