癌变·畸变·突变 ›› 2007, Vol. 19 ›› Issue (1): 33-035.doi: 10.3969/j.issn.1004-616x.2007.01.010

• 论著 • 上一篇    下一篇

两种人类肝细胞系评价饮水氯化消毒副产物MX致 DNA损伤作用的敏感性

吴建军1；刘爱林2；邹亚玲2；周利红2；鲁文清2   

  1. 1.广州医学院化学致癌研究所, 广州　510182; 2.华中科技大学同济医学院公共卫生学院环境卫生教研室, 湖北　武汉　430030
  • 收稿日期:2005-10-17 修回日期:2006-02-28 出版日期:2007-01-30 发布日期:2007-01-30

Susceptibility of Two Human-Derived Hepatic Cell Lines in Evaluating DNA Damage Induced by MX

WU Jian-jun1, LIU Ai-lin2, ZOU Ya-ling2, ZHOU Li-hong2, LU Wen-qing2   

  1. 1.Institute for Chemical Carcinogenesis of Guangzhou Medical College, Guangzhou 510182; 2. Department of Occupational and Environmental Health, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
  • Received:2005-10-17 Revised:2006-02-28 Online:2007-01-30 Published:2007-01-30

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摘要： 背景与目的: 研究两种人类来源的肝细胞在评价饮水氯化消毒副产物3-氯-4-二氯甲基-5-羟基-2(5氯)-呋喃酮(MX) DNA损伤作用的敏感性。材料与方法: 应用单细胞凝胶电泳试验(彗星试验), 研究人肝癌细胞株HepG2和人胚肝细胞L-02在评价饮水氯化消毒副产物MX DNA损伤作用的敏感性。 结果: 随着MX剂量增加，可引起HepG2(>30 μmol/L)和L-02细胞(>100 μmol/L)DNA迁移度显著增加；中度以上损伤所占百分比在同剂量组中HepG2细胞大于L-02细胞，经比较分析，差异有统计学意义(χ2=20.985；8.0；11.97，P<0.05)。 结论： 应用单细胞凝胶电泳试验评价MX的DNA损伤作用，人肝癌细胞株HepG2比人胚肝细胞L-02更为敏感。

关键词: HepG2, L-02, 单细胞凝胶电泳试验, DNA损伤

Abstract: BACKGROUND ＆ AIM: To study on the susceptibility of two human-derived hepatic cell lines in evaluating DNA damage induced by 3-chloro-4-dichloromethyl-5-hydroxy-2(5H)-furanone(MX). MATERIALS AND METHODS: Using single cell gel electrophoresis assay, to evaluate the DNA damage induced by MX in human hepatocellular carcinoma cell lines(HepG2) and human fetal hepatocytes (L-02). RESULTS: MX could induce HepG2 and L-02 DNA single strand breaks at the concentrations of 30 and 100 μmol/L, respectively. The length of DNA migration induced by MX increased significantly in comparison to solvent control (DMSO) in a dose-response manner(P<0.05). Moderate or greater DNA damages induced by MX in HepG2 were more serious than that of L-02(χ2=20.985；8.0；11.97， P<0.05). CONCLUSION: HepG2 was more susceptible than L-02 cells to DNA damage induced by MX evaluated by using single cell gel electrophoresis assay.

Key words: HepG2, L-02, single cell gel electrophoresis assay, DNA damage

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