关键词: 莱菔硫烷, 细胞周期, P16, P27

Abstract: 【ABSTRACT】 BACKGROUND ＆AIM： To study the effects and mechanisms of sulforaphane on T24 cell cycle. MATERIALS AND METHODS： The inhibitory effects of sulforaphane on the growth of T24 cells were investigated and its value of IC50 after 72 h incubation were measured by 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl tetrazolium bromide(MTT) method. The effect of sulforaphane on the T24 cell cycle was determined by flow cytometry; and expressions of P27 and P16 induced by sulforaphane were studied by western blotting analysis. RESULTS： Sulforaphane in low concentrations (≤40 μmol/L)could significantly suppress the growth of T24 cells in a dose-dependent way.The inhibitory rates for 10 μmol/L,20 μmol/L and 40 μmol/L were (12.5±3.95)％，(25.0±2.50)％ and (50.0±5.33)％,respectively. High concentrations of sulforaphane(60 μmol/L－160 μmol/L), the inhibitory effects showed no obvious dose-dependency. The IC50 value of sulforaphane treatment for 72 h was (51.12±7.10)μmol/L. Sulforaphane could block T24 cell cycle at the G0/G1 phase as showed by flow cytometry. Moreover, the treatment of 20 μmol/L sulforaphane for 48 h caused the appearance of pre-G1 before G0/G1 phase. Treatments with 20 μmol/L sulforaphane for 8, 12, 24 h significantly induced the expression of P27 protein in a time-dependent manner. At early stage of sulforaphane treatment(8 h) ,the expression of P16 protein was also induced. CONCLUSION： Sulforaphane could inhibit the growth of T24 cells and block cell cycle at G0/G1 phase. The induction of P27 protein by sulforaphane mainly involved molecular mechanisms, may be including the early induction of P16 protein.

Key words: Sulforaphane, Cell cycle, P16, P27