癌变·畸变·突变 ›› 2010, Vol. 22 ›› Issue (5): 343-345.doi: 10.3969/j.issn.1004-616x.2010.05.004

• 论著 • 上一篇    下一篇

肝门部胆管癌细胞系FRH 0201与正常肝细胞系L02蛋白质组学差异分析

刘　博1,肖雪媛2,何大澄2,黄志强1   

  1. 1. 中国人民解放军总医院肝胆外科,北京 100853； 2. 北京师范大学生命科学学院，高校蛋白质组学研究院，北京 100875
  • 收稿日期:2010-03-15 修回日期:2010-05-30 出版日期:2010-09-30 发布日期:2010-09-30
  • 通讯作者: 刘　博

Analysis of differential expression of proteins in portal cholangiocarcinoma cell line FRH 0201 and hepatocellular cell line L02

LIU Bo1, XIAO Xue-yuan2 ,HE Da-cheng2, HUANG Zhi-qiang1   

  1. 1. Department of Hepatobiliary Surgery,General Hospital of People′s Liberation Army, Beijing 100853; 2. College of Life Sciences, Universty Institute for Proteomics, Beijing Normal University, Beijing 100875,China)
  • Received:2010-03-15 Revised:2010-05-30 Online:2010-09-30 Published:2010-09-30
  • Contact: LIU Bo

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摘要： 目的： 分析肝门部胆管癌细胞系FRH 0201与正常肝细胞系L02的蛋白质表达差异，筛选肝门部胆管癌的潜在分子标志物。 方法： 应用表面增强激光解吸离子化(surface enhanced laser desorption/ionization，SELDI)蛋白质芯片技术检测肝门部胆管癌及正常肝细胞系的蛋白质谱。用PBSII_C型蛋白质芯片阅读机读取数据，采用Proteinchip软件分析数据。 结果： IMAC3、WCX2两种蛋白芯片共捕获 144 个蛋白峰，发现16个差异蛋白。与正常肝细胞系L02蛋白谱相比，4个蛋白在肝门部胆管癌细胞系FRH 0201中高表达，12个蛋白在肝门部胆管癌细胞系FRH 0201中低表达。 结论： 肝门部胆管癌与正常肝细胞存在差异蛋白表达，这些差异蛋白有可能成为潜在的肝门部胆管癌标志物，对其进行研究有助于了解肝门部胆管癌的发病机制。

关键词: 肝门部胆管癌, 肝细胞, 差异蛋白

Abstract: OBJECTIVE: To analyze the differential expression of proteins in portal cholangiocellular carcinoma cell line FRH 0201 and normal hepatocellular cell line L02, and to screen potential molecular markers for diagnosis of hilar cholangiocarcinoma. METHODS: Surface enhanced laser desorption/ionization (SELDI) mass spectrometry with Proteinchip IMAC3 and WCX2 was performed to compare the expressed proteins in portal cholangiocellular carcinoma cell line FRH 0201 and hepatocellular cell line L02. Protein profiling was examined by PBSII_C Protein Chip Reader and the proteome database was analyzed by Proteinchip Software 3.0.2. RESULTS: 144 protein peaks in FRH 0201 and L02 cell lines were captured by Proteinchip IMAC3 and WCX2，16 differential proteins were found. Four proteins were up_regulated and 12 proteins down_regulated in portal cholangiocellular carcinoma cell line FRH 0201 as compared with L02 cell line. CONCLUSION: The differential proteins could be candidate biomarkers of portal cholangiocarcinoma. The study may contribute to understanding the mechanisms of development of portal cholangiocarcinoma

Key words: portal cholangiocarcinoma, hepatocyte, differential protein

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