癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (4): 237-239.doi: 10.3969/j.issn.1004-616x.2003.04.016

• 检测研究 • 上一篇    下一篇

白眉蝮蛇蛇毒精氨酸酯酶的致突变研究

韦传宝1,2，余茂耘2   

  1. 1.浙江大学生命科学学院，浙江　杭州　310027；2.皖西学院生物系，安徽　六安　237000
  • 收稿日期:2002-10-23 修回日期:2003-05-10 出版日期:2003-10-30 发布日期:2003-10-30
  • 通讯作者: 韦传宝

STUDY ON THE MUT AGENICITY OF ARGININE ESTERASE FROM AGKISTRODON HALYS USSURIENSIS VENOM

WEI Chuan-bao , YU Mao-yun   

  1. 1.College of Life Science, Zhejiang University, Hangzhou 310027, China；2.Biology Department,Wanxi College,Liuan 27300,China
  • Received:2002-10-23 Revised:2003-05-10 Online:2003-10-30 Published:2003-10-30
  • Contact: WEI Chuan-bao

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摘要： 目的与方法：采用Ames试验、小鼠骨髓嗜多染红细胞微核试验及小鼠精子畸形试验研究白眉蝮蛇(Agkistrodon halys ussuriensis )蛇毒中精氨酸酯酶有无致突变毒性，为临床应用提供安全性依据。结果与结论：3项试验均为阴性，精氨酸酯酶对所试菌株、小鼠体细胞及雄性生殖细胞均无诱变性。从致突变角度考虑，精氨酸酯酶在高于临床治疗剂量约100倍的条件下仍然安全。

关键词: 精氨酸酯酶, Ames试验, 微核试验, 精子畸形试验

Abstract: Purpose and methods : The potential mutageni city of arginine esterase from Agkistrodon halys ussuriensis venom was studied by Ames test, micronu cleus test of polychromatic erythrocytes in bone marrow and sperm shape abnormality test in mice to evaluate its safety for clinical application. Results and conclusion: The results of all the three testes were negative. It was suggested that the arginine esterase is not mutagenic to the tested strains, to the somatic cells and to the male germ cells. If Its gene mutagenesis was considered as an important factor, arginine esterase is still safe at the dosage of about 100 times clinical dosage.

Key words: arginine esterase, Ames test, micronucleus test, sperm shape abnormality test

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