广州某茶叶市场普洱茶中多种生物毒素污染现状调查

doi:10.3969/j.issn.1004-616x.2011.01.018

›› 2011, Vol. 23 ›› Issue (1): 68-071.doi: 10.3969/j.issn.1004-616x.2011.01.018

广州某茶叶市场普洱茶中多种生物毒素污染现状调查

陈建玲1,2,李文学1,杨光宇1,周志涛3,陈雯2,朱伟1*,刘华章1*

1. 广州市疾病预防控制中心，广东广州 510080； 2. 中山大学公共卫生学院，广东广州 510080；3. 南方医科大学生物技术学院，广东广州 510515

收稿日期:2010-09-01 修回日期:2010-12-01 出版日期:2011-01-30 发布日期:2011-01-30
通讯作者: 朱伟

Bioligical contamination of Pu'er tea in a Guangzhou tea market

HEN Jian-ling1,2,LI Wen-xue1,YANG Guang-yu1,ZHOU Zhi-tao3, CHEN Wen2, ZHU Wei1,*,LIU Hua-zhang1,*

1. Guangzhou Center for Diseases Control and Prevention, Guangzhou 510080; 2. School of Public Health, Sun Yat-sen University, Guangzhou 510080; 3. School of Biotechnology, Southern Medical University, Guangzhou 510515, China

Received:2010-09-01 Revised:2010-12-01 Online:2011-01-30 Published:2011-01-30
Contact: ZHU Wei

摘要/Abstract

摘要： 为了解广州某茶叶市场湿仓储存普洱茶中真菌毒素的污染情况，对所抽取的70份普洱茶样本中黄曲霉毒素B1(aflatoxin B1，AFB1)、伏马毒素(fumonisin B1，FB1)、呕吐毒素(脱氧雪腐镰刀菌烯醇，deoxynivalenol，DON)和T-2毒素的浓度水平进行检测，旨在为制定有关普洱茶叶安全卫生管理规范和相关的卫生标准提供数据资料。方法：随机抽取广州某茶叶市场湿仓储存普洱茶70份，破碎、浸提、过滤后采用免疫亲和层析净化-高效液相色谱法检测AFB1，ELISA试剂盒检测FB1、DON和T-2毒素的污染情况。结果：本次调查所采70份普洱茶样品中， AFB1污染水平超出标准规定限值(5 μg/kg)的有8例(11.43%)， DON污染水平超出标准规定限值(1 mg/kg)的有 63例(90％)，FB1和T-2毒素污染水平均未超出标准规定限值(分别为1 mg/kg和100 μg/kg)。结论：本调查结果提示广州市该茶叶市场中的湿仓储存普洱茶存在不同程度的AFB1及DON污染情况，而FB1及T-2毒素虽未超出标准规定的限值，但在样品中均可检出，应引起广泛重视。

关键词: 普洱茶, 黄曲霉毒素, 伏马毒素, 呕吐毒素, T-2毒素

Abstract: To understand the level of bioligical contamination by toxins including aflatoxinB1(AFB1), FumonisinB1(FB1), DON and T-2 toxins of Pu'er tea in a tea market of Guangzhou,China, to provide useful information and data for safety and related health standards. METHODS: Sample 70 Pu'er tea randomizely in a Guangzhou tea market. All the samples were assayed with ELISA kit for FB1, DON, T-2 toxin, and with immunoaffinity chromatography - high performance liquid chromatography for AFB1. RESULTS: The AFB1 level of 8 among 70 samples was above the limit of national standard(5 μg/kg), and the DON level of 63 samples above the national standard(1 mg/kg), while the levels of FB1 and T-2 toxin were within the standards(1 mg/kg and 100 μg/kg，respectively). CONCLUSION: AFB1 and DON in our samples were higher and exceeded the allowed standards to some degree. Althouth the levels of FB1 and T-2 were within eimits below, their existence in Pu'er tea should require close attention .

Key words: Pu’er tea, aflatoxin B1, fumonisin B1, deoxynivalenol, T-2 toxins

陈建玲1,2,李文学1,杨光宇1,周志涛3,陈雯2,朱伟1*,刘华章1*. 广州某茶叶市场普洱茶中多种生物毒素污染现状调查[J]. , 2011, 23(1): 68-071.

HEN Jian-ling1,2,LI Wen-xue1,YANG Guang-yu1,ZHOU Zhi-tao3, CHEN Wen2, ZHU Wei1,*,LIU Hua-zhang1,*. Bioligical contamination of Pu'er tea in a Guangzhou tea market[J]. , 2011, 23(1): 68-071.

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[2]	李志芳,李道传,王庆,刘彩霞,李文学,陈雯*. 黄曲霉毒素B1诱导的恶性转化肝细胞miRNA表达谱的变化[J]. , 2011, 23(1): 26-030.
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广州某茶叶市场普洱茶中多种生物毒素污染现状调查

Bioligical contamination of Pu'er tea in a Guangzhou tea market

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