Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (1): 57-061.doi: 10.3969/j.issn.1004-616x.1998.01.019

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THE METHODOLOGY ON THE MULTINUCLEATED CELL ASSAY

Wu Hongliang1 , Zhu Wei1 , Zheng Siying2 , Gao J insheng1   

  1. 1 Department of radiation biology , 2 Department of biology , S uz hou Medical College , S uz hou   215007
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-01-30 Published:1998-01-30

Abstract: Three main factors influcing the multinucleated cell assay were evaluated with the L9 (3) 4 orthogonal design. The result was the best when the 6 - TG final density was 2 ×10 - 5M , the Cyt - B final density was 6mg/ l , and the culture time was 72 hours. Meanwhile the other factors were also studied.

Key words: the multinucleated cell assay, methodology, orthogonal design, hprt gene locus variant f requency