挥发性物质体外致突变定量检测方法的研究

doi:10.3969/j.issn.1004-616x.1998.03.017

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (3): 182-187.doi: 10.3969/j.issn.1004-616x.1998.03.017

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A METHOD OF THE QUANTITATIVE MEASUREMENT OF THE MUTAGENICITY OF VOLATILE SUBSTANCE IN VITRO

Zhou Yonggui1 , Yu Qingping2 , Fan Yulan1 , Li Zhaoming2 , You Xuejun1

S hanghai Instit unte of L abour Hygiene and Occupational Disease , S hanghai \ 200003 　 JiangNan S hi pyard , S hanghai 　200011

Received:1900-01-01 Revised:1900-01-01 Online:1998-05-30 Published:1998-05-30

Abstract

Abstract: A new sealed container for testing the mutagenicity of the volatile shbstance is designed and simple , repid and useful mutagenic quantitative method for these substances in vit ro is developed. The media/ air partition cofficient s and the concent ration in the container of the volatile ubstance are measured using the equilibration Princile. Then the concent ration of the substance tested in the media is got by simple formule. The whole procedure is completed during 4 hrs only. The given comcent ration of the volatile substance in the container can be kept , and the dose2effect relationship is clear. In Ames/ Salmenlla assay the result s suggested that , when volatile chemicals with the boiling Point of below 100 ℃are tested the mutagenic test must use the container ; when the chemicals with the boiling point of 100 ℃- 200 ℃are tested the container is still useful and elected firstly.

Key words: volatile substance, Mutation, Quantitative Mesaurement s

Zhou Yonggui , Yu Qingping , Fan Yulan , Li Zhaoming , You Xuejun. A METHOD OF THE QUANTITATIVE MEASUREMENT OF THE MUTAGENICITY OF VOLATILE SUBSTANCE IN VITRO[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1998, 10(3): 182-187.

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A METHOD OF THE QUANTITATIVE MEASUREMENT OF THE MUTAGENICITY OF VOLATILE SUBSTANCE IN VITRO

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