应用等位基因特异性PCR 和多重差别PCR 检测肺癌患者CYP1A1 和GSTM1 的遗传多态性

doi:10.3969/j.issn.1004-616x.1999.03.005

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1999, Vol. 11 ›› Issue (3): 119-122.doi: 10.3969/j.issn.1004-616x.1999.03.005

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IDENTIFICATION OF GENETIC POLYMORPHISM OF CYP1A1 AND GSTM1 IN LUNG CANCER PATIENTS BY USING ALLELE-SPECIFIC PCR AND MULTIPLEX DIFFERENTIAL PCR

Chen Senqing , Xu Lin , Ma Guojian , Wu J ianzhong , Xue Kaixian

Jiangsu Instit ute of Cancer Research , Nanjing 　210009

Received:1999-02-28 Revised:1999-03-12 Online:1999-05-30 Published:1999-05-30

Abstract

Abstract: Allele2specific PCR(AS2PCR) and multiplex differential PCR (MD2PCR) were used to identify the genotypes of CYP1A1 and GSTM1 in 68 lung cancer patients and 105 healthy cont rols. The result s showed that genotypes of CYP1A1 and GSTM1 could be detected simultaneously with AS2PCR and MD2PCR ; The frequency of CYP1A12Val/ Val in lung cancer patient s group was 12/ 68 (17. 6 %) , about 2. 052fold than that in healthy cont rols 9/ 105 (8. 57 %) , and the f requency of GSTM1 0/ 0 was 39/ 68 (57. 4 %) , remarkably higher compared with 42/ 105 (40 %) in healthy cont rols ( P < 0. 05) 1

Key words: allele-specific PCR, multiplex differential PCR, polymorphism, CYP1A1, GSTM1

Chen Senqing , Xu Lin , Ma Guojian , Wu J ianzhong , Xue Kaixian. IDENTIFICATION OF GENETIC POLYMORPHISM OF CYP1A1 AND GSTM1 IN LUNG CANCER PATIENTS BY USING ALLELE-SPECIFIC PCR AND MULTIPLEX DIFFERENTIAL PCR[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1999, 11(3): 119-122.

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IDENTIFICATION OF GENETIC POLYMORPHISM OF CYP1A1 AND GSTM1 IN LUNG CANCER PATIENTS BY USING ALLELE-SPECIFIC PCR AND MULTIPLEX DIFFERENTIAL PCR

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