Abstract: Purpose : To develop a inducible system for expression of Trail in E. coli . Methods : RT2PCR and sequencing were used to clone and conform Trail gene. The production of expression was analyzed by SDS2page.Results : Trail cDNA was cloned by RT2PCR. After sequencing , we const ructed the inducible vector for expression of Trail in E. coli . The vector was t ransformed with TG1. Trail was expressed by induced of changing temperature f rom 30 ℃to 42 ℃. The production was conformed by SDS2page. Conclusion : This study will provide new insight into physiological and pathological roles of Trail2mediated cytotoxicity and it s potential application to tumor immunotherapy.

Key words: Trail, gene cloning, gene expression