FISH技术评价昆明山海棠在小鼠骨髓细胞中的非整倍体诱发效应

doi:10.3969/j.issn.1004-616x.2002.03.002

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (3): 144-146.doi: 10.3969/j.issn.1004-616x.2002.03.002

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ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRI PTERYGIUM HYPOGLAUCUM ( LEVEL ) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH

DING Yin-run, WANG Xiao-yan, WANG Xu

Genetic laboratory, School of Life Sciences, Yunnan Normal University, Kunming 650092, China

Received:2001-07-24 Revised:2001-09-30 Online:2002-07-30 Published:2002-07-30
Contact: WANG Xu

Abstract

Abstract: Purpose : The water ext ract of a Chinese herb , Tri pterygium hypoglaucum ( Level ) Hutch ( THH)was assayed for its effects of aneuploidy induction in mouse bone marrow cells by means of fluorescence in situ hybridization (FISH) . Methods : Kunming species mice were injected with THH into the abdominal cavity and killed at 24 h after t reatment . The bone marrow cell slides were prepared by general methods. FISH was carried out with Bio2162dU TP2labbled chromosome 8 probe. The hybridization signals were detected by combining st reptavidine-Cy3. Results : In three dose groups THH-induced chromosome 8 aneuploidy and the aneuploidy frequencies of chromosome 8 were significantly higher than the solvent cont rol ( P < 0. 001～0. 05) . Conclusion : The result indicated that THH is an aneugen of chromosome 8 in mouse somatic cells.

Key words: fluorescence in situ hybridization, Tripterygium hypoglaucum (Lvel ) Hutch, mouse bone marrow cell, aneuploidy

CLC Number:

R99. 4

DING Yin-run, WANG Xiao-yan, WANG Xu. ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRI PTERYGIUM HYPOGLAUCUM ( LEVEL ) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2002, 14(3): 144-146.

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ANEUPLOIDY INDUCTION BY THE WATER EXTRACT OF TRI PTERYGIUM HYPOGLAUCUM ( LEVEL ) HUTCH IN MOUSE BONE MARROW CELLS DETECTED BY FISH

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