Carcinogenesis, Teratogenesis & Mutagenesis ›› 2002, Vol. 14 ›› Issue (3): 135-138.doi: 10.3969/j.issn.1004-616x.2002.03.001

    Next Articles

MUTATIONS AND EXPRESSION CHANGES OF DNA-PK GENES DETECTED IN THE MALIGNANT TRANSFORMED BEP2D CELLS INDUCED BY α-PARTICLES EXPOSURE

YANG Tao,SUI Jian-li.GENG Yu,YANG Su-xia,ZHOU Ping-kun,WU De-chang   

  1. 1. Department of Radiation Toxicology,eijing Institute of Radiation Medicine.Beijing 100850, China;2. Institute of Naval Medicine, Shanghai 200433
  • Received:2002-01-04 Revised:2002-02-05 Online:2002-07-30 Published:2002-07-30
  • Contact: ZHOU Ping-kun

PDF (PC)

2659

Abstract: Purpose : To detect the mutations and expression changes of DNA-PK genes in the t ransformed human bronchial epithelial cells (BEP2D) induced by α-particles. Methods : Mutations were detected with PCRSSCP ; gene expression was analyzed using Northern blot hybridization. Results : Mutations in the encoding sequence of Ku70 (XRCC-6) gene were discovered in the malignant transformed cells. In the process of α-particle induced BEP2D transformation , the expression of DNA2PKcs gene was depressed at the early stage of t ransformation , and was up2regulated again in some of the malignant t ransformant s. Conclusion : The mutations and depressed expression of DNA repair genes lead to the deficiency of DNA DSBs repair capacity , which in turn might result in genomic instability. These events could play an important role in the malignant transformation of BEP2D cells induced byα-particles exposure.

Key words: human bronchial epithelial cell line BEP2D, α-particles, carcinogenesis, DNA2PK genes, DNA repair

CLC Number: