Abstract: BACKGROUND ＆ AIM: Human semen cryopreservation is an important link of assisted reproductive techniques.After human sperm storeroom was approved by the government,human cryopreservative spermatozoa was widely used in assisted reproductive techniques. It has become more important to study on chromosome aberration of human cryopreservative spermatozoa.In this report,we analyze the chromosome aberration of human cryopreservative spermatozoa brought by CPM. MATERIAL AND METHODS:Firstly, we classified the CPM into four groups.First group：glycerol-egg yolk-sodium citrate(G-Y-C)；second group：glycerol-honey(G-H)； third group： glycerol-HEPES-hty(G-H-H)；fourth group：glycerol-tyrede(T-G).Secondly,the mixture of spermatozoa and CPM were done with slow freezing method.Finally,after half a year,we analyzed the human chromosome aberration. We took it out to come back to normal temperoture in 37 ℃ water. RESULTS:Rate of chromosomal breakage and rate of aberration had not striking increased among the four groups. CONCLUSION: The four groups,sacceded with CPM are trust able in clinical application.

Key words: spematozoa, cryopreservative medium(CPM), spermatozoa chromosome