应用基因表达谱芯片研究MNNG诱致小鼠胚胎畸形肢体基因表达的变化

doi:10.3969/j.issn.1004-616x.2005.02.002

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (2): 71-75.doi: 10.3969/j.issn.1004-616x.2005.02.002

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Investigation on the Gene Expression Pattern of Abnormal Limb of Mouse Embryo Following Exposure to MNNG

GUO Miao-li;ZHU Jiang-bo;CHEN Rong-fang;ZHANG Tian-bao

Department of Military Hygiene,Second Military Medical University, Shanghai 200433, China

Received:2004-05-21 Revised:2004-07-14 Online:2005-03-30 Published:2005-03-30
Contact: ZHANG Tian-bao

Abstract

Abstract: BACKGROUND ＆ AIM: To analyse the gene expression profiles in abnormal and normal developmental limbs of GD14 embryo mice. MATERIAL AND METHODS: A series of expression microarray analysis of abnormal limb were initiated by cDNA microarray which representing a set of 8 192 mice genes. RESULTS: By applying this cDNA microarray we identified 287 differentially expressed genes, among which 73 upregulated and 214 downregulated. CONCLUSION: cDNA microarray for analysis of gene expression patterns is a powerful method to identify teratogenicity-related gene. Further analysis of these differentially expressed genes will be helpful for understanding the molecular mechanism of teratogenicity.

Key words: N-methy-N'-nitro-N-nitrosoguandine, gene expression profiles, abnormal limb, cluster analysis, mouse

GUO Miao-li, ZHU Jiang-bo, CHEN Rong-fang, ZHANG Tian-bao. Investigation on the Gene Expression Pattern of Abnormal Limb of Mouse Embryo Following Exposure to MNNG[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(2): 71-75.

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Investigation on the Gene Expression Pattern of Abnormal Limb of Mouse Embryo Following Exposure to MNNG

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