Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (3): 239-242.doi: 10.3969/j.issn.1004-616x.2007.03.020

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The Expression of CAGE Gene and Its Mechanism in Intracranial Tumors

GAO Fei1, YOU Tian2, LU Jing-yu3, WANG Yun-jie4   

  1. 1. Department of Neurosurgery, the Fourth Clinical College, China Medical University，Shenyang 110032；2. Department of Emergency Medicine,the First Clinical College, China Medical University，Shenyang 110001；3. Department of Medical Genetics, China Medical University，Shenyang 110001；4. Department of Neurosurgery, the First Clinical College, China Medical University, Shenyang 110001,China
  • Received:2006-06-12 Revised:2006-10-08 Online:2007-05-30 Published:2007-05-30
  • Contact: LU Jing-yu

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Abstract: BACKGROUND ＆ AIM: We studied the expression and promoter methylation of CAGE gene in intracranial tumors in order to explore its potential use in tumor immunotherapy. MATERIALS AND METHODS: RT-PCR and methylation-specific PCR were performed to detect the expression of CAGE gene in 17 normal tissues, 32 glioma and 35 meningioma, as well as the pattern of methylation in the promoter region. RESULTS: The CAGE gene was expressed in none of the normal tissues except testis. It was expressed in 4 meningioma (11.3％) and 26 glioma (81.25％). We found PCR products with methylation-specific primers in all the CAGE-negative cases and the PCR products with non-methylation-specific primers in all the CAGE-positive cases. CONCLUSION: The methylation of CAGE gene was closely associated with its expression. CAGE could act as the tumor-specific antigen for glioma.

Key words: CAGE gene, methylation, glioma, tumor immunity

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