Abstract: BACKGROUND ＆ AIM: To investigate the antioxidation effects of Panax capsule with grape seed extract(GSE) on old rats with different doses . MATERIALS AND METHODS: Three different doses(0.1、0.5、2.5 g/kg) and a control group(were dosed through mouth with solvent only) were administered through mouth to three experiment groups,respectively, once per day for 60 days. On the 61 day, All the rats were killed through vein exsanguinate.The blood and the liver were obtained from the rats and were disposed, respectively.The content of serum MDA ,activation of GSH_Px and SOD were detected. RESULTS: The results showed no significant differences between the three experiment groups and the control group about the activation of SOD(P>0.05). However, there were significant differences between the experiment group (2.5 g/kg) and the control group both about the content of serum MDA and the activation of GSH_Px . CONCLUSION: Panax capsule with GSE could decrease the content of serum MDA of the old female rats (age 15 monthes) and increase the activation of GSH_Px. It can be concluded that Panax capsule with GSE has the effect of antioxidation.

Key words: grape seed extract, antioxidation, MDA, GSH_Px, SOD