Carcinogenesis, Teratogenesis & Mutagenesis ›› 2008, Vol. 20 ›› Issue (5): 354-358.doi: 10.3969/j.issn.1004-616x.2008.05.005

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Expression of UDP_glucuronosyl_transferase 2B and Hepatic Nuclear Factor 1α in Colorectal Cancer Tissues

ZHANG Yan, LI Yan_qing, ZHANG Shang_zhong, YANG Xiao_yun, YUAN Jun_hua, GUO Yu_ting, LU Xue_feng   

  1. Department of Gastroenterology of Qilu Hospital, Shangdong University, Jinan 250012, Shangdong,China
  • Received:2007-10-15 Revised:2008-03-10 Online:2008-09-30 Published:2008-09-30

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Abstract: BACKGROUND AND AIM: To evaluate the expression of UDP_ glucuronosyltransferase (UGT) 2B subfamily and hepatic nuclear factor1α(HNF1α)in colorectal cancer, and to explore its significance in colorectal tissue carcinogenesis. MATERIALS AND METHODS: RT_PCR was used to determine the mRNA levels of four UGT isoforms and hepatic nuclear factor1α(HNF1α) in 32 samples of colorectal cancer and 14 normal colorectal sambles.UGT2B protein was measured by western blot analysis and immunochemistry was used for HNF1α protein. RESULTS: Polymorphic regulation of UGT2B isoforms expression was found in colorectal tissue; compared to control group, UGT2B4 mRNA level of the 32 samples of colorectal cancer and its surrounding healthy tissue was not significantly different(P>0.05). UGT2B7,UGT2B15 mRNA level were significantly down_regulated in colorectal cancer and its surrounding healthy tissues when compared to control group(P<0.05). UGT2B7 protein level was significantly decreased in colorectal cancer and its surrounding healthy tissues(P<0.05). Compared to control group, mRNA and protein levels of HNF1α were not significantly changed in colorectal cancer and its surrounding healthy tissues(P>0.05). CONCLUSION: Decreased expression level of UGT isoform may be one cause of colorectal tissue carcinogenesis. As an upstream regulator of UGT genes, mRNA and protein levels of HNF1α were not significantly changed. This may be related to complicated control mechanisms in vivo.

Key words: colorectal cancer, UDP_glucuronosyltransferase, hepatic nuclear factor1α