Abstract: BACKGROUND AND AIM: To investigate the effects of overexpression of the 24-amino-acid N-terminal end of p55γ regulatory subunit of phosphoinositide-3 kinase on proliferation and migration mediated by adenovirus in MGC803 gastric carcinoma cells. MATERIALS AND METHODS: The recombinant adenovirus-Ad-N24-GFP and control virus Ad-GFP were amplified in HEK293 cells. The virus infection rate of tumor cells was determined by fluorescence microscope. The effects of Ad-N24-GFP on cell proliferation and cell cycle were detected by cell growth curve and flow cytometry. The effect of Ad-N24-GFP on cell migration was detected by Transwell migration assay. RESULTS: The infection rate of recombinant adenovirus of MGC803 cells was highest when MOI=100. Compared with control cells, the growth of MGC803 cells after Ad-N24-GFP infection was suppressed and cell doubling time was prolonged. The percentage of Ad-N24-GFP cells in G0/G1 phase was (84.2±5.4)％, significantly higher than that of control cells in G0/G1 phase (68.7±5)％ (P<0.05). Overexpression of the Ad-N24-GFP fusion protein markedly resulted in decrease of MGC803 cell migration compared with the control cells (P<0.05). CONCLUSION: Overexpression of the p55 γ gene N-terminal 24 peptide mediated by adenovirus inhibited cell proliferation and migration in gastric carcinoma MGC803 cells. It could have potential application in gastric carcinoma gene therapy.

Key words: adenovirus, p55γ, gastric carcinoma, cell proliferation, migration