›› 2011, Vol. 23 ›› Issue (3): 206-208.doi: 10.3969/j.issn.1004-616x.2011.03.011

• 论著 • Previous Articles     Next Articles

Gene mutation of 15 STR loci analysis of the IdentifilerTM system in parentage testing

ZHANG Dan-yan1， ZHANG Dan-mei2，WAN Ling1，LU Jing1，CHEN Xiao-xing1，ZHAO Le-tian1，LI Lian-bing1   

  1. 1. Science and Technology Research Institute of Population and Family Planning in Chongqing City / Chongqing Zheng-ding Judicial Identification Institute/Key Laboratory of Birth Defects and Reproductive Health of Chongqing, Chongqing 400020; 2. Chongqing Normal University，Chongqing 401331, China
  • Received:2011-01-13 Revised:2011-03-13 Online:2011-05-30 Published:2011-05-30
  • Contact: ZHANG Dan-yan

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Abstract: Observation and analysis of gene mutation of 15 short tandem repeat (STR) loci of the IdentifilerTM system in paternity testing. METHODS：Make use of the IdentifilerTM fluorescent multiplex PCR kit for indentification of 710 paternity cases. If the gene mutant was found we would be perform STRtyper fluorescent multiplex PCR kit or six mini Y-STR loci for detection of alleles. RESULTS： In the 615 cases identified in the parent-child relationship， seven cases of mutation were found in IdentifilerTM fluorescent multiplex PCR kit detection， of which 2 cases at vWA loci，and one each at D13S17， FGA， D18S51， D21S11 and D19S433 loci； step mutation in the six cases， two-step mutation in 1 case. Mutations were found in the father， aged over 35 years. CONCLUSION： 1-2 STR loci found are not in line with the expected by fluorescent multiplex PCR IdentifilerTM kit， it is necessary to increase STR loci with low mutation rate and good stability to confirm and to exclude consanguinity.

Key words: genetics, parentage testing, STR loci, gene mutation