Carcinogenesis, Teratogenesis & Mutagenesis
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XU Wen-li，ZHANG Wei，QIN Xiu-jun，YUE Juan，LI Shu-fang，WANG Yong-li，WEN Jian-hua*
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OBJECTIVE: To investigate the safety of intravenous injection of allogeneic adipose-derived mesenchymal stem cells (ADSCs) in rats after acute radiation. METHODS：A total of 45 SPF male SD rats were equally and randomly divided into three groups：blank control group，radiation control group and radiation grafting group. Rat ADSCs were isolated and cultured in vitro. Irradiated control group and irradiated grafting rats were received 7 Gy dose total body irradiation once. Rats in each group received injection via tail vein，with 10 mL/ kg of 1×106 ADSCs as the irradiated grafting group and the equal volume physiological saline as blank control group and irradiated control group. The rats were observed for 34 days for their general reactions before they were sacrificed. Blood samples were collected for hematological studies at the 16th day and biochemical studies at the 34th day. Visceral organs were inspected and studied pathologically. RESULTS：No significant difference in weight，hematological and biochemical studies was detected between radiation grafting and radiation control groups(P<0.05). No significant difference was found in histopathological examination in the visceral organs among blank control，radiation control and radiation grafting groups，no appearance of acute and chronic graft versus host disease was obsessed. CONCLUSION：Intravenous infusion of allogeneic mesenchymal stem cells was feasible and safe. No side effects were found in recipients.
Key words: adipose-derived mesenchymal stem cells, acute radiation sickness, stem cell transplantation, security
XU Wen-li，ZHANG Wei，QIN Xiu-jun，YUE Juan，LI Shu-fang，WANG Yong-li，WEN Jian-hua. Safety of rat adipose-derived mesenchymal stem cell transplantation in the treatment of acute radiation sickness[J]. Carcinogenesis, Teratogenesis & Mutagenesis, doi: 10.3969/j.issn.1004-616x.2013.06.008.
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