真菌纤溶化合物1的遗传毒性评价

doi:10.3969/j.issn.1004-616x.2023.03.011

Abstract

Abstract: OBJECTIVE：To perform a systematic genotoxicity assessment of fungal fibrinolytic compound 1 (FGFC1) in an established GLP laboratory platform. METHODS：A combination of classical genotoxicity assays (Ames，in vitro culture CHO cell chromosome aberration and mouse bone marrow micronucleus assays) was used to detect genotoxicity of FGFC1. RESULTS：Results from the Ames assay indicate that FGFC1 was not mutagenic to Salmonella typhimurium at five doses of 0.5，5，50，500 and 1 000 g per dish，with and without the addition of the S9 metabolic activation system. FGFC1 did not induce chromosomal aberrations in the presence of S9 after 24 h and 48 h of treatments. The rates of micronucleus induction in bone marrow cells at 3 doses of 62.5，125.0 and 250.0 mg/kg was not significantly different from that in the solvent control group (P>0.05). CONCLUSION：The above results indicate that FGFC1 was not genotoxic and not potentially carcinogenic under the test conditions.

Key words: fungal fibrinolytic compound, Ames test, chromosomal aberration test, micronucleus assay, genetic toxicity

CLC Number:

R394.6

SHI Wenjing, TIAN Yijun, DONG Yachun, WU Wenhui, ZHANG Tian, ZHANG Huishu, ZHU Yuping. Evaluation of genotoxicity of fungal fibrinolytic compound 1[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2023, 35(3): 219-223.

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Evaluation of genotoxicity of fungal fibrinolytic compound 1

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