SPP1、DEC1、C1QTNF6蛋白与口腔鳞状细胞癌患者临床病理指标及预后的关系

doi:10.3969/j.issn.1004-616x.2024.02.004

Abstract

Abstract: OBJECTIVE： To investigate correlations of serum secreted phosphoprotein 1 (SPP1)， differentiated embryo-chonrocyte expressed gene 1 (DEC1) and the complement C1q/tumor necrosis factor associated protein 6 (C1QTNF6) levels with clinicopathologic outcomes of oral squamous cell carcinomas. METHODS： Immunohistochemical staining and electrochemiluminescence immunoassay were used to detect protein expression levels of SPP1，DEC1 and C1QTNF6 in tumor tissues and serum samples from 88 patients with oral squamous cell carcinoma. Pearson correlation and Kaplan-Meier survival analyses were used to analyze correlations between protein expression levels of serum SPP1， DEC1 and C1QTNF6 and clinicopathological outcomes. Multivariate Cox regression analysis was used to analyze the risk factors affecting the prognosis of patients with oral squamous cell carcinoma. RESULTS：Results from immunohistochemical staining showed that positive expression levels of SPP1， DEC1 and C1QTNF6 proteins in carcinoma tissues were 1.94， 2.98 and 2.35 times higher than those in adjacent normal tissues (P<0.05 or P<0.01). Results from the electrochemiluminescence immunoassay showed that expression levels of serum SPP1，DEC1 and C1QTNF6 in patients were up-regulated by 8.61，6.20 and 4.03 times (P<0.05 or P<0.01) compared with normal controls. Pearson correlation analysis results showed that expression levels of serum SPP1，DEC1 and C1QTNF6 were positively correlated with the occurrence of multifocal neurotropic invasion， degree of tumor invasion， lymph node metastasis，and higher TNM stage (P<0.05). Kaplan-Meier survival analysis showed that patients with high serum SPP1， DEC1 and C1QTNF6 protein expression levels had a low overall survival rate. Multivariate Cox regression analysis showed that multifocal neurotropic invasion，high tumor invasion，lymph node metastasis and high TNM stage were the risk factors for prognosis of patients (P<0.05). CONCLUSION：Abnormally elevated serum SPP1， DEC1 and C1QTNF6 proteins in patients were positively correlated with multifocal perineural invasion， tumor invasion， lymph node metastasis and higher TNM stage， but negatively correlated with the prognosis. Systematic detection of expression levels of SPP1， DEC1 and C1QTNF6 in serum of patients have important value and significance for mastering patient's conditions and for guiding clinical practices.

Key words: oral squamous cell carcinoma, secreted phosphoprotein 1, differentiated embryo-chonrocyte expressed gene 1, the complement C1q/tumor necrosis factor associated protein 6, prognosis

CLC Number:

R730.2

FU Yongqing, XU Sanhui, ZHAO Yan, WANG Lili. Relationships between SPP1, DEC1, C1QTNF6 and clinicopathologic outcomes of oral squamous cell carcinomas[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(2): 107-111,117.

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Relationships between SPP1, DEC1, C1QTNF6 and clinicopathologic outcomes of oral squamous cell carcinomas

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