脂肪酸代谢相关基因ACSL1在急性髓系白血病中的作用及潜在价值

doi:10.3969/j.issn.1004-616x.2024.04.009

Abstract

Abstract: OBJECTIVE：To analyze involvement of the fatty acid metabolism-related gene，long-chain esteryl-CoA synthetase 1 (ACSL1)，in acute myeloid leukemia (AML) through bioinformatics analysis. METHODS：Expression levels of the ACSL1 gene in AML patients and healthy controls were analyzed using the Gene Expression Profiling Interaction Analysis (GEPIA) database. The Kaplan-Meier survival curves for the ACSL1 high and low expression groups were plotted by using R-package survminer，and correlations between the ACSL1 gene expression and the survival rate of AML patients were analyzed. The receiver operating characteristic (ROC) curve of ACSL1 was plotted using the timeROC package in R language to explore the diagnostic efficiency of ACSL1 gene in controls and AML patients. Single gene enrichment analysis (GSEA) was used to clarify the oncogenic and lipid metabolism pathways of ACSL1 gene. Kaplan-Meier survival analysis was used to determine relationships between ACSL1 expression level and overall survival (OS). Univariate and multivariate Cox regression analysis were used to establish a prognostic model and to determine whether ACSL1 was an independent prognostic factor for AML，and to investigate the prediction effect of the model. RESULTS：The expression levels of ACSL1 in AML patients were significantly higher than those in the controls (P<0.05)，and they had high diagnostic significance for the AML patients. In addition，patients in the ACSL1 high expression group had poor survival and poor prognosis (P=0.045). The ROC curve showed that the area under the curve (AUC) of ACSL1 in diagnosing the 1-，2-，and 3-year survival time of AML patients was greater than 0.6，indicating that the prediction accuracy of ACSL1 was high. Enrichment analysis showed that ACSL1 played an important role in the occurrence and development of AML，mainly through fatty acid degradation and metabolism. Univariate Cox analysis showed that age and ACSL1 expression level were high-risk factors affecting the overall survival rate of AML patients (HR>1，P<0.05)，and multivariate Cox analysis showed that age，chromosomal abnormality score and gene mutation score were independent factors affecting the prognosis of AML patients (P<0.05). CONCLUSION：ACSL1，a fatty acid metabolism-related gene，is highly expressed in AML，and enriched in multiple oncogenic and fatty acid metabolism pathways. Its high expression was closely related to poor prognosis and，therefore，the expression can be used as a potential therapeutic target and prognostic biomarker for AML.

Key words: acute myeloid leukemia, fatty acid metabolism, ACSL1, prognostic analysis, bioinformatics

CLC Number:

R733.3

CUI Jian, CHEN Ye, HE Li, BAO Ying, DONG Shaojuan, ZHU Danxia, WU Jun, CUI Mengying. Involvement of the fatty acid metabolism-related gene ACSL1 in acute myeloid leukemia[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2024, 36(4): 298-304.

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Involvement of the fatty acid metabolism-related gene ACSL1 in acute myeloid leukemia

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