全血接种量及培养体系对淋巴细胞核质桥分析的影响

doi:10.3969/j.issn.1004-616x.2025.05.009

Abstract

Abstract: OBJECTIVE： To investigate effects of whole blood inoculation and culture systems on formation of nucleoplasmic bridges (NPB) and on formation of radiation-induced NPB，for providing scientific basis for radiation biological dosimeters. METHODS：Human venous blood and fingertip blood were cultured as follows：400，300，200，100 and 50 μL of venous blood were inoculated into 1 or 2 mL medium，100 μL of fingertip blood were inoculated into 1 mL medium. These cultures were irradiated with 0 Gy (control group) and 2 Gy ⁶⁰Co γ radiation at a dose rate of 1.0 Gy/min. NPB specimens were prepared by cytokinesis-block method. The proportion of mononucleated，binucleated and multinucleated cells，as well as the NPB and micronucleus (MN) frequencies of each group were analyzed. RESULTS：In the 0 Gy group， 1 048~9 768 binucleated cells were analyzed in different blood inoculation groups. The NPB frequency did not change significantly with the whole blood inoculation (P>0.05). The MN frequency in the 50 μL group was higher than that in the other three groups (U=3.61，P<0.01). In the 2 Gy group，there was no cell available for analysis in the 50 μL group，and 1 569-4 400 binucleated cells were analyzed in other blood inoculation groups. The NPB frequency was 0.009-0.013 cells/cell，and the MN frequency was 0.317-0.337 cells/cell，and there was no significant difference with the whole blood inoculum (P > 0.05). The NPB and MN frequencies of the 2 Gy group were significantly higher than those of the 0 Gy group (U=4.24-34.48， P<0.05). Fingertip blood culture failed to observe cells for analysis. CONCLUSION： The minimum 50 μL and 100 μL peripheral blood culture combined with 1 mL culture medium can be used to study the background level of the nucleoplasmic bridge and the radiation-induced nucleoplasmic bridge level respectively. The feasibility of fingertip blood culture for nucleoplasmic bridge analysis still needs further study.

Key words: nucleoplasmic bridge, whole blood inoculum, cytokinesis-block, micronucleus

CLC Number:

R994.6

ZHAO Hua, LU Xue, CAI Tianjing, QI Xuesong, LIU Qingjie. Effect of whole blood inoculum and culture system on lymphocyte nucleoplasmic bridges formation[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2025, 37(5): 400-404.

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Effect of whole blood inoculum and culture system on lymphocyte nucleoplasmic bridges formation

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