纺锤体毒剂和断裂剂体外诱发V79 细胞多核与微核效应的差异

doi:10.3969/j.issn.1004-616x.1998.05.004

Carcinogenesis, Teratogenesis & Mutagenesis ›› 1998, Vol. 10 ›› Issue (5): 282-285.doi: 10.3969/j.issn.1004-616x.1998.05.004

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DIFFERENTIAL EFFECTS OF ANEUGENS AND CLASTOGENS ON INCIDENCES OF MULTINUCLEATED CELLS AND OF MICRONUCLEATED CELLS IN CHINESE HAMSTER LUNG ( V79 ) CELL LINE IN VITRO

Liu Yungang , Wu Zhongliang , and Chen J iakun

Instit ute f or Chemical Carcinogenesis , Guangz hou Medical College , Guangz hou 510182

Received:1900-01-01 Revised:1900-01-01 Online:1998-09-30 Published:1998-09-30

Abstract

Abstract: Two aneugens : vinblastine (Vin) and colchicine (Col) ,and two clastogens : mitomycin C (MMC) and cyclophosphamide (CP) , were applied to Chinese hamster lung (V79) cells respectively in vit ro for micronucleus test . The result s showed that the increases of incidence of multinucleated cells ( Imu) and that of micronucleated cells ( Imi ) in response to aneugens and clastogens were in different sensitivities. At relatively low concent rations , Vin and Col induced only Imu (but not Imi) , while MMC induced only Imi ; At higher concent rations , both Imu and Imi increased in a concent ration dependent manner , however , Vin and Col induced multinuclei predominantly with the ratios of Imu to Imi 2. 0 - 3. 5 ,while MMC and CP induced micronuclei mainly , with Imu/ Imi of 0. 08 - 0. 28. This intensity of difference in Imu/ Imi was much greater than that in areas of micronuclei between aneugens and clastogens. The result s indicate that in micronucleus test in vit ro , observation of both Imu and Imi might be valuable for distinguishing aneugens f rom clastogens.

Key words: Aneugen, Clastogen, Multinucleus, Micronucleus

Liu Yungang , Wu Zhongliang , and Chen J iakun. DIFFERENTIAL EFFECTS OF ANEUGENS AND CLASTOGENS ON INCIDENCES OF MULTINUCLEATED CELLS AND OF MICRONUCLEATED CELLS IN CHINESE HAMSTER LUNG ( V79 ) CELL LINE IN VITRO[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1998, 10(5): 282-285.

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DIFFERENTIAL EFFECTS OF ANEUGENS AND CLASTOGENS ON INCIDENCES OF MULTINUCLEATED CELLS AND OF MICRONUCLEATED CELLS IN CHINESE HAMSTER LUNG ( V79 ) CELL LINE IN VITRO

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