葡多酚对肝癌细胞增殖活性与Ca2＋浓度的影响

doi:10.3969/j.issn.1004-616x.2006.04.009

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (4): 289-291.doi: 10.3969/j.issn.1004-616x.2006.04.009

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Effects of Grape Procyanidins on the Proliferative Activity and the Concentration of Intracellular Calcium of Hepatoma Cells

ZHANG She-hua, LI jie, LIU Hui, ZHONG Jin-yi

Institute of Nutrition,Medical College of Qingdao University,Qingdao 266021,Shandong,China

Received:2005-09-25 Revised:2006-02-26 Online:2006-07-30 Published:2006-07-30
Contact: ZHONG Jin－yi

Abstract

Abstract: BACKGROUND ＆ AIM: To investigate the effects of grape procyanidins on the proliferative activity and the concentration of intracellular calcium of hepatoma cells. MATERIAL AND METHODS: Human hepatoma cells were cultured with differrent concentrations of GPC. The proliferative activity and concentration of intracellular calcium of the hepatoma cells were measured by methyl thiazolyl tetrazolium(MTT) assay and microfluorimetric techniques based on calcium indicator Fura-2/AM. RESULTS: The proliferation level and concentration of intracellular calcium of the control group were (0.045±0.024) nmol/L and (130.53±17.48) nmol/L, respectively. The cell proliferation activitives of 3 GPC groups were lower, but the concentration of intracellular calcium was higher with increasing amounts of GPC(t>2.78, P<0.05). The concentration of intracellular calcium of both 50 mg/L and 100 mg/L GPC group were overloaded. CONCLUSION:GPC could inhibit the proliferative activity and abnormally elevate the concentration of intracellular calcium of hepatoma cells .

Key words: grape procyanidins(GPC), hepatoma cells, proliferation activity, concentration of intracellular calcium

CLC Number:

735.1

ZHANG She-hua, LI jie, LIU Hui, ZHONG Jin-yi. Effects of Grape Procyanidins on the Proliferative Activity and the Concentration of Intracellular Calcium of Hepatoma Cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2006, 18(4): 289-291.

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Effects of Grape Procyanidins on the Proliferative Activity and the Concentration of Intracellular Calcium of Hepatoma Cells

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