Abstract: Purpose : The mitotic effects of d-tubocurarine in V79 cells and mouse bone marrow cells was investigated in this study. d2t ubocurarine is a antagonist to cholinergic receptors and inhibitor of nicotinic receptor. Methods : V79 Chinese hamster cells were t reated in vit ro by d2tubocurarine. The alteration of late telophaseearly G1 configuration induced by this chemical was determined by analyzing of presence or lack of a cleavage furrow in late telophase and very early G1 cells , the abnormal cytokinesis was investigated for the f requencies of binuclear cells in interphase cells. Simultaneously , we investigated the C2M effect s of d2t ubocurarine in mouse bone marrow cells. Results : The tested chemical disturbed mitotic coordination and significantly induced high frequency of binuclear cells in V79 cell line ( P < 0. 001) . There is no other distinguished abnormality in mouse bone marrow cells but significant enhance of C2M cells at low dose group. Conclusion : The result s indicated that d-tubocurarine is able to inhibit mammal mitosis by blocking the function of nicotinic receptor. The malfunction of cholinergic and nicotinic receptors may be a mechanism of aneuploid induction.

Key words: V79 hamster cell, mitosis, aneuploidy