采用单细胞凝胶电泳技术检测断裂剂对精子DNA损伤

doi:10.3969/j.issn.1004-616x.2005.01.010

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2005, Vol. 17 ›› Issue (1): 30-32.doi: 10.3969/j.issn.1004-616x.2005.01.010

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Detection of DNA Damage in Human ＆ Mouse Sperm by Modified SCGE

SONG Bo;CAI Zhi-ming;ZHENG Lu-kang2, et al

Departemnt of Andrology,Shenzhen Hospital of Peking University,Shenzhen 518036, China

Received:2004-03-21 Revised:2004-07-05 Online:2005-01-30 Published:2005-01-30
Contact: SONG Bo

Abstract

Abstract: BACKGROUND ＆ AIM: To assess whether the modified SCGE can be used in genetic toxicology. MATERIAL AND METHODS: To detect DNA damage induced by oxidant peroxide in human sperm in vitro and methyl methanesulfonate in mouse in vivo by using the modified SCGE. RESULTS: The results showed high relationship correlation among the head DNA percentage,comet length and the tail moment.Dose response relationship existed. CONCLUSION: The modified SCGE could be used to detect sperm DNA damage.

Key words: sperm, DNA, SCGE

SONG Bo, CAI Zhi-ming, ZHENG Lu-kang, et al. Detection of DNA Damage in Human ＆ Mouse Sperm by Modified SCGE[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2005, 17(1): 30-32.

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Detection of DNA Damage in Human ＆ Mouse Sperm by Modified SCGE

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