热休克预处理可抑制N_甲基_N＇_硝基_N_亚硝基胍对CHL细胞的遗传毒性

doi:10.3969/j.issn.1004-616x.2007.02.002

Carcinogenesis, Teratogenesis & Mutagenesis ›› 2007, Vol. 19 ›› Issue (2): 89-092.doi: 10.3969/j.issn.1004-616x.2007.02.002

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Pre-heat Pre-heat Shock Protects CHL Cells from the Genotoxicity of N-methyl-N＇-nitro-N-nitrosoguanidine

DONG Zheng-wei1,HU Hu2,LIU Guang-yi1,SHEN Xiao-jun1,YANG Jun1,

1.Institute of Toxicology, School of Public Health, Zhejiang University;2.Department of Pathology ＆ Pathophysiology, Zhejiang University, Hangzhou 310058,Zhejiang,China

Received:2006-11-06 Revised:2006-12-07 Online:2007-03-30 Published:2007-03-30
Contact: YANG Jun

Abstract

Abstract: BACKGROUND ＆ AIM: To study whether heat shock could induce the formation of γH2AX foci in CHL cells, and the effect of heat shock on N-methyl-N＇-nitro-N-nitrosoguanidine(MNNG)-induced γH2AX foci formation. MATERIALS AND METHODS: The cytotoxic effect of heat shock was evaluated by MTT test.The phosphorylation of γH2AX was assessed using immunofluorescent microscopy and flow cytometry. RESULTS: MTT test showed that heat shock decreased cell viability.There was no significant difference in the number of γH2AX foci between heat-shock treated group and control group as shown by immunofluorescent microscopy. Flow cytometry analysis revealed that pre-heat shock followed by MNNG treatment decreased the mean γH2AX fluorescent intensity from 0.316±0.042 in the MNNG-treatment alone group to 0.194±0.011. CONCLUSION: Heat shock did not induce γH2AX foci formation in CHL cells.In addition, heat shock could inhibit the phosphorylation of H2AX induced by MNNG.

Key words: heat shock, γH2AX, DNA damage, genotoxicity

CLC Number:

R994.6

DONG Zheng-wei, HU Hu, LIU Guang-yi, SHEN Xiao-jun, YANG Jun, . Pre-heat Pre-heat Shock Protects CHL Cells from the Genotoxicity of N-methyl-N＇-nitro-N-nitrosoguanidine[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2007, 19(2): 89-092.

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Pre-heat Pre-heat Shock Protects CHL Cells from the Genotoxicity of N-methyl-N＇-nitro-N-nitrosoguanidine

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