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Effect of Periplocin Extracted from Cortex Periplocae on Proliferation of Colorectal Cancer Cell SW480 in Vitro and in Vivo DU Yan-yan, LIU Shi-bin, LIU Xin, SHAN Bao-en Carcinogenesis, Teratogenesis & Mutagenesis    2009, 21 (3): 181-184.   DOI: 10.3969/j.issn.1004-616x.2009.03.006 Abstract （2999）      PDF （9194KB）（19520）       Knowledge map    Save BACKGROUND AND AIM: The inhibition effect of periplocin extracted from cortex periplocae (CPP) on proliferation of colorectal cancer cell line SW480 was studied in vitro and in vivo. MATERIALS AND METHODS: The inhibitiory effects of different concentrations of CPP (0.125,0.25,0.5,1.0,2.0 μg/ml) on the growth of SW480 cells in vitro were measured by MTT assay. The cell morphology and microstructural changes of apoptosis were examined under light microscope and electron microscope after SW480 cells were treated with CPP(0.5 μg/ml) for 24 h. Survivin expression in SW480 cells was analyzed by immunohistochemistry after cells were treated with CPP (0.5 μg/ml) for 24 h. Model of transplanted tumor on nude mouse were used to study the anticancer effect in vivo. Control group was included in all experiments above. RESULTS: CPP significantly inhibited the proliferation of SW480 cells in a dose- and time-dependent manner in vitro (P<0.01). After treatment with CPP, SW480 cells showed some typical morphologic features and microstructural changes of apoptosis. And the expression of Survivin in SW480 cells was suppressed. After treatment with CPP 30 mg/kg for SW480 cell tumor-bearing mice, the inhibition rate of transplanted tumor was significantly increased at 61.41％. Inflammatory cells and noticeable necrosis were found in transplanted tumor tissue of CPP treated group. CONCLUSION: CPP had significant inhibitory effect on colorectal cancer cell line SW480. The mechanism maybe associated with CPP inhibiting the expression of Survivin in SW480 cells and inducing cell apoptosis. Related Articles | Metrics

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Expressing the alteration of DNA repair genes in malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate LI Ying1，WANG Quan-kai1，2，WANG An-na1，2，HU Jie1，XU Jian-ning1，2，* null    2011, 23 (3): 161-165.   DOI: 10.3969/j.issn.1004-616x.2011.03.001 Abstract （3097）      PDF （926KB）（10180）       Knowledge map    Save To observe the expression change of malignant transformation- related DNA repair genes in human bronchial epithelial cells (16HBE) induced by glycidyl methacrylate (GMA). METHODS：The expression of DNA repair genes in 30th generation cells which had malignant cell phenotype was evaluated with the gene chip of “human genome stability/DNA repair microarray”. RESULTS：Compared with control cells in the 30th generation，eight genes screened from 113 genes showed different expressions in cells induced by GMA. Among the eight genes，there were 7 genes (NBN，RAD50，RAD51，OGG1，XAB2，TOP3A，TNKS) with increased expression (ratio>2)and 1 gene (NEIL2) with lower expression (0 Related Articles | Metrics

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Inhibitory effects of DVDMS-2-based-photodynamic therapy on the growth of tumor in vitro and in vivo JIANG Zhi-huan,SHI Rui1,LI Chao2,WANG Ai-ping Carcinogenesis, Teratogenesis & Mutagenesis    DOI: 10.3969/j.issn.1004-616x.2013.03.001

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DOSE-RESPONSE RELATIONSHIP BETWEEN CELL PROL IFERATION AND HYDROGEN PEROXIDE Carcinogenesis, Teratogenesis & Mutagenesis    1997, 09 (2): 92-99.   DOI: 10.3969/j.issn.1004-616x.1997.02.006 Abstract （1533）      PDF （226KB）（5123）       Knowledge map    Save Related Articles | Metrics

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null    2011, 23 (2): 148-151.   DOI: 10.3969/j.issn.1004-616x.2011.02.016 Abstract （2496）      PDF （387KB）（4937）       Knowledge map    Save Related Articles | Metrics

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Carcinogenesis, Teratogenesis & Mutagenesis    2008, 20 (5): 412-414.   DOI: 10.3969/j.issn.1004-616x.2008.05.019 Abstract （2428）      PDF （141KB）（4607）       Knowledge map    Save Related Articles | Metrics

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null    2012, 24 (5): 393-396.   DOI: 10.3969/j.issn.1004-616x.2012.05.019 Abstract （2898）      PDF （433KB）（4567）       Knowledge map    Save Related Articles | Metrics

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RAPID DETECTION OF ob GENOTYPE BY PCR AND RESTRICTION ENDONUCLEASE XU Pei-yu , Jerlin Walker Carcinogenesis, Teratogenesis & Mutagenesis    2000, 12 (3): 143-145.   DOI: 10.3969/j.issn.1004-616x.2000.03.005 Abstract （2449）      PDF （74KB）（4336）       Knowledge map    Save Purpose and Methods : The ob gene point mutant in C57BL/ 6J ob mice cause genetic obesity in all life. To improve the speed and efficiency of ob genotyping , we developed a method with PCR and rest riction endonuclease by property primer. Results and Conclusion : Three genotype (ob/ ob , ob/ + , + / + ) of the mouse are rapidly , differetiated with this assay. Since only a small biopsy specimen ( tail end) is needed to genotype ,the ob mouse at any age is typable and can be used in subsequent breeding or research. Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    2001, 13 (2): 122-129.   DOI: 10.3969/j.issn.1004-616x.2001.02.018 Abstract （1804）      PDF （116KB）（4308）       Knowledge map    Save Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    2001, 13 (1): 55-057.   DOI: 10.3969/j.issn.1004-616x.2001.01.019 Abstract （2265）      PDF （83KB）（4245）       Knowledge map    Save Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    1999, 11 (3): 158-160.   DOI: 10.3969/j.issn.1004-616x.1999.03.016 Abstract （1571）      PDF （74KB）（4181）       Knowledge map    Save Related Articles | Metrics

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EXPRESSION OF WILMS’TUMOR GENE( WT1) IN LEUKEMIA AND ITS CLINICAL IMPLICATION Zhou Hailan , Yu Kang , Chen Yi Carcinogenesis, Teratogenesis & Mutagenesis    1999, 11 (5): 222-224.   DOI: 10.3969/j.issn.1004-616x.1999.05.004 Abstract （2585）      PDF （91KB）（4143）       Knowledge map    Save To elucidate the expression of Wilms’tumor gene (WT1) in various types of leukemia and its clinical implication. WT1 mRNA was detected in K562 cell line , 23 leukemia patient s (12AML patient , 3 ALL patient , 7 CML patient , 1 CLL patient . ) 2 lymphoma patient s in three stage , and 10 healthy subjects by using RT2PCR. WT1 mRNA expression was revealed in K562 cell line , 10 of 12 AML patient s , 2 of 3 ALL patients , 6 CML patient s in blast crisis. WT1 mRNA was undetecatable in 1 CML patient in accelerated , 1 CLL patient , 2 NHL in three phase and 10 healthy subject s. WT1 gene is expressed in early hematopoietic cells , it is closely related with acute leukemia , and supposed to be a new marker for monitoring MRD in acute leukemia. Related Articles | Metrics

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Oxidative Effect of H2O2 on HepG2 Cells in Different Phases of the Cell Cycle GAO Zhi-qing, HAI Chun-xu, LIANG Xin, QIN Xu-jun, ZHANG Xiao-di, LIU Rui Carcinogenesis, Teratogenesis & Mutagenesis    2007, 19 (2): 122-124.   DOI: 10.3969/j.issn.1004-616x.2007.02.012 Abstract （2863）      PDF （177KB）（4139）       Knowledge map    Save BACKGROUND ＆ AIM： To study the oxidative effect of H2O2 on HepG2 cells in different phases of the cell cycle to explore the apoptosis mechanism of cancer cells under oxidative stress. MATERIALS AND METHODS：Synchronized cells were collected by arresting with TdR, then cells at different phases of cell cycle were treated by 800 μmol/L H2O2 and content of MDA, activities of XOD, 5'NT , anti- O2 and anti-•OH were measured. RESULTS： The content of MDA and activities of XOD, anti-•OH,anti- O2 increased and activities of 5'NT decreased significatly in cells treated with H2O2 . Compared with non-synchronized cells, the content of MDA and activities of XOD, 5'NT，anti-•OH and anti- O2 increased markedly in synchronized S-phase cells and G2/M-phase cells .The content of MDA and activities of XOD, 5'NT， anti- O2 were significantly lower in synchronized G1-phase cells. CONCLUSION： When synchronized cells were treated with H2O2, the degree of lipid peroxidation, activities of enzyme that catalyse production of free radicals， and level of anti-oxidation were all associated with the cell cycle. This may be due to different sensitivities to oxidative damage induced by H2O2 at the different phases of the cell cycle. Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    1999, 11 (2): 0-0.   DOI: 10.3969/j.issn.1004-616x.1999.02.001 Abstract （2189）      PDF （61KB）（4059）       Knowledge map    Save Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    1999, 11 (3): 144-146.   DOI: 10.3969/j.issn.1004-616x.1999.03.012 Abstract （2058）      PDF （75KB）（3970）       Knowledge map    Save Related Articles | Metrics

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Carcinogenesis, Teratogenesis & Mutagenesis    2008, 20 (1): 79-080.   DOI: 10.3969/j.issn.1004-616x.2008.01.019 Abstract （2522）      PDF （134KB）（3956）       Knowledge map    Save Related Articles | Metrics

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Carcinogenesis, Teratogenesis & Mutagenesis    2010, 22 (6): 484-487.   DOI: 10.3969/j.issn.1004-616x.2010.06.019 Abstract （2030）      PDF （316KB）（3940）       Knowledge map    Save Related Articles | Metrics

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REVIEW Carcinogenesis, Teratogenesis & Mutagenesis    2002, 14 (1): 54-057.   DOI: 10.3969/j.issn.1004-616x.2002.01.019 Abstract （1887）      PDF （82KB）（3894）       Knowledge map    Save Related Articles | Metrics

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Present Contamination Status of Water Organic Pollutants and Parts of Persistent Organic Pollutants in China QIU Zhi-qun, SHU Wei-qun, CAO jia, Carcinogenesis, Teratogenesis & Mutagenesis    2007, 19 (3): 188-194.   DOI: 10.3969/j.issn.1004-616x.2007.03.007 Abstract （3404）      PDF （324KB）（3894）       Knowledge map    Save This article introduces the contamination status of organic pollutants in water environment of China, and also the character and polluted situation of persistent organic pollutants (POPs) such as polychlorinated biphenyl, dioxin and phthalates. Additionally, some suggestions about further research on POPs have been proposed. Related Articles | Metrics

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Research Progress of Heavy Metals in Water Environment of China WANG Yan, HUANG Yu-ming Carcinogenesis, Teratogenesis & Mutagenesis    2007, 19 (3): 198-202.   DOI: 10.3969/j.issn.1004-616x.2007.03.009 Abstract （3762）      PDF （251KB）（3885）       Knowledge map    Save This paper summarizes the current research status of heavy metals in water body of China. It is emphasized that heavy metals' appearance, migration and transfer regularity and toxicity studies are also expounded. Related Articles | Metrics

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Clinical Implications of Microsatellite Instability in Diagnosis of Bladder Cancer Recurrence DI Jin-ming, LI Qing-wen, GUAN Chao, ZHU Yong-xian, LIU Jian-min, ZHOU Wen-sheng, HU Yong-quan Carcinogenesis, Teratogenesis & Mutagenesis    2005, 17 (5): 308-310.   DOI: 10.3969/j.issn.1004-616x.2005.05.015 Abstract （2702）      PDF （548KB）（3880）       Knowledge map    Save BACKGROUND ＆ AIM: To evaluate the value and potential clinical implications of microsatellite instability(MSI) in diagnosis of bladder cancer recurrence. MATERIAL AND METHODS: The analysis by PCR for detecting MSI was done in 20 cases of nomal samples and 60 cases of urine samples from 42 patients with bladder carcinoma in postoperative follow-up. The analytic parameters included hematuresis ,tumor size, number of tumor, tumor grade and 5 microsatellite bio-markers. RESULTS: There were 17 cases of MSI＋ in 20 recurrent cases, of which 15 cases got hematuresis samples. The sensitivity, specificity and positive predictive value was 85.00％, 82.50％ and 83.33％, respectively, with the analysis by the results of MSI in diagnosis of bladder cancer recurrence. No correlation was found between MSI and tumor grading. CONCLUSION: The results indicate that MSI is a good bio-marker in diagnosis of bladder cancer recurrence for patients with bladder carcinoma in postoperative follow-up, especially for which with hematuresis. Related Articles | Metrics

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REVIEW Carcinogenesis, Teratogenesis & Mutagenesis    2002, 14 (2): 131-134.   DOI: 10.3969/j.issn.1004-616x.2002.02.021 Abstract （2100）      PDF （81KB）（3830）       Knowledge map    Save Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    1998, 10 (2): 119-122.   DOI: 10.3969/j.issn.1004-616x.1998.02.016 Abstract （2010）      PDF （80KB）（3787）       Knowledge map    Save Related Articles | Metrics

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review Carcinogenesis, Teratogenesis & Mutagenesis    2007, 19 (2): 162-164.   DOI: 10.3969/j.issn.1004-616x.2007.02.025 Abstract （1877）      PDF （143KB）（3730）       Knowledge map    Save Related Articles | Metrics

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MUTATION STUDY OF P53 GENE IN PATIENTSW ITH LARGE BOWEL CARCINOMA Carcinogenesis, Teratogenesis & Mutagenesis    1997, 09 (4): 199-202.   DOI: 10.3969/j.issn.1004-616x.1997.04.001 Abstract （1585）      PDF （202KB）（3726）       Knowledge map    Save Related Articles | Metrics

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Carcinogenesis, Teratogenesis & Mutagenesis    2002, 14 (1): 47-049.   DOI: 10.3969/j.issn.1004-616x.2002.01.017 Abstract （2103）      PDF （219KB）（3708）       Knowledge map    Save Related Articles | Metrics

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Review Carcinogenesis, Teratogenesis & Mutagenesis    2006, 18 (6): 491-493.   DOI: 10.3969/j.issn.1004-616x.2006.06.022 Abstract （2143）      PDF （166KB）（3708）       Knowledge map    Save Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    1999, 11 (2): 109-112.   DOI: 10.3969/j.issn.1004-616x.1999.02.018 Abstract （2080）      PDF （80KB）（3707）       Knowledge map    Save Related Articles | Metrics

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ANALYSIS OFP53 GENE ALTERATION IN HUMAN LUNG CANCER BaiLi, Sun Yan, Li Shende Carcinogenesis, Teratogenesis & Mutagenesis    1996, 08 (6): 321-326.   DOI: 10.3969/j.issn.1004-616x.1996.06.001 Abstract （2184）      PDF （233KB）（3701）       Knowledge map    Save Related Articles | Metrics

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Experimental Study on Apoptosis Induced by Ginsenoside Rg3 in Human CF-7 Breast Cancer Cell Line CHEN Jun-xia, XIA Jun, LIU Ji-wei, CUI Xiu-yun Carcinogenesis, Teratogenesis & Mutagenesis    2005, 17 (4): 213-216.   DOI: 10.3969/j.issn.1004-616x.2005.04.006 Abstract （2796）      PDF （701KB）（3684）       Knowledge map    Save BACKGROUND ＆ AIM: Ginsenoside Rg3 is a kind of effective chemical trace component extracted from the red panix. Many studies demonstrated that ginsenoside Rg3 might block angiogenesis and inhibit tumor growth and metastasis. The mechanism of ginsenoside Rg3 in anti-tumor is now one of hot point of chinese traditional medicine researches. The purpose of this study is to explore the effect of Rg3 on induction apopotosis of human MCF-7 breast cancer cell line. MATERAIL ＆ METHODS: The cell proliferation inhibition was measured by 3-[4,5-dimethylthiazo-2-yl]-2,5 diphenyl tetrazolium bromide(MTT) assay. The morphological changes of cells were observed by fluorescence staining of Hoechst 33258. Cell cycle and apoptosis rate were analyzed by flow cytometry. DNA ladder was showed by agarose gel electrophoresis. RESULTS： There was obviously a concentration-dependent relationship between Rg3 and inhibition of the cell proliferation，r=0.953, (P<0.05). IC50 was 71.91 μg/ml Rg3 after treatment for 48 hours. The cells treated with 150 μg/ml Rg3 for 48 hours showed morphological characteristics of apoptotic cells with the condensed chromatin, the nuclear fragmentation, the apoptotic body and brighter green fluorescent. FCM assay indicated that Rg3 regulated cell cycle and induced cell apoptosis. The percentage of cells in S phase and G2-M phase was increased, whereas the percentage of cells in G0-G1 was decreased. The apoptosis rates were increased from(0.45±0.25) ％ to(34.86±4.65)％ in MCF-7 cells after treatment with Rg3 for 48 hours. The cells treated with 150 μg/ml Rg3 for 24 and 48 hours reveal an obvious DNA ladder. CONCLUSION： The results suggested that Ginsenoside Rg3 exerts an inhibiting effect on human MCF-7 breast cancer cell line by inducing apoptosis of the cells. Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    2002, 14 (4): 253-255.   DOI: 10.3969/j.issn.1004-616x.2002.04.015 Abstract （2349）      PDF （77KB）（3676）       Knowledge map    Save Related Articles | Metrics

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Carcinogenesis, Teratogenesis & Mutagenesis    1996, 08 (6): 377-381.   DOI: 10.3969/j.issn.1004-616x.1996.06.014 Abstract （1987）      PDF （117KB）（3667）       Knowledge map    Save Related Articles | Metrics

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REVIEW Carcinogenesis, Teratogenesis & Mutagenesis    2002, 14 (2): 127-130.   DOI: 10.3969/j.issn.1004-616x.2002.02.020 Abstract （1578）      PDF （85KB）（3662）       Knowledge map    Save Related Articles | Metrics

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HOMOZYGOUS DEFICIENCY OF HUMAN GLUTATHIONE STRANSFERASE T1 GENE IN NORMAL POPULATION IN SHANGHAI Lin Guofang , Tan J ingwei , Shen J ianhua Carcinogenesis, Teratogenesis & Mutagenesis    1998, 10 (6): 345-348.   DOI: 10.3969/j.issn.1004-616x.1998.06.004 Abstract （2643）      PDF （98KB）（3636）       Knowledge map    Save Human glutathione S2t ransferase ( GSTs) is considered as an important group of phase reaction enzymes involved not only in the detoxification metabolism ,in some cases , also in the bioactivation process of various types of environmental hazard chemicals. Among the glutathione S2t ransferases known so far , GSTT1 displays genetic polymorphism due to gene deletion. GSTT1 was genotyped in 226 healthy adults in Shanghai (permanent resident s) by an internal control allele2specific PCR amplification of blood nucleated cell DNA. The homozygous deletion of gene ( GSTT10/ 0 genotype) was identified in 50. 3 %(114/ 226) of the individuals tested. The deficiency frequency in normal population in Shanghai is higher than in normal Caucasian population (11 - 17 %) and among Af rican Americans (24 %) significantly. Related Articles | Metrics

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STUDY OF MITOTIC EFFECTS OF ATROPINE IN V79 CHINESE HAMSTER CELLS He Zhijun , Wang Xu Carcinogenesis, Teratogenesis & Mutagenesis    1999, 11 (3): 125-127.   DOI: 10.3969/j.issn.1004-616x.1999.03.007 Abstract （2737）      PDF （181KB）（3611）       Knowledge map    Save V79 Chinese hamster cells were treated in vit ro by at ropine sulfate , which is an antagonist to cholinergic receptors and inhibitor of muscarinic receptor. The alteration of late telophase2early G1 configuration induced by at ropine were determined by analyzing present or lack of a cytokinesis furrow in late telophase and very early G1 cells , The abnormal cytokinesis were investigated for the f requency of binuclear cells in interphase cells.The tested chemical disturbed mitotic coordination and induced significantly high f requency of binuclear cells.The result indicated that at ropine is able to inhibit mammal mitosis by blocking the function of cholinergic receptor. The malfunction of cholinergic receptors may be a mechanism of aneuploid induction. Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    1999, 11 (1): 54-057.   DOI: 10.3969/j.issn.1004-616x.1999.01.020 Abstract （1462）      PDF （83KB）（3605）       Knowledge map    Save Related Articles | Metrics

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Carcinogenesis, Teratogenesis & Mutagenesis    1994, 06 (3): 33-37.   DOI: 10.3969/j.issn.1004-616x.1994.03.010 Abstract （2004）      PDF （284KB）（3599）       Knowledge map    Save Related Articles | Metrics

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EFFECTS OF VARIOUS ANTIOXIDANTS ON UV2INDUCED THE FORMATION OF HUMAN SKIN'S CYCLOBUTYL PRIMIDINE DIMERS E Xiaofei , Zhu Xudong , Li J ing , Liu Yang , Sun Xinyan , Wang Bingxian Carcinogenesis, Teratogenesis & Mutagenesis    1998, 10 (6): 331-335.   DOI: 10.3969/j.issn.1004-616x.1998.06.001 Abstract （2467）      PDF （154KB）（3568）       Knowledge map    Save To define further the role of reactive oxygen species ( ROS) in ult raviolet radiation (UV)2induced the formation of Human skin’s CPD , experiment s were performed using alkaline gelelect rophoresis technique. Purified DNA was incubated with antioxidant s. After DNA was exposed to UV , T4 Endonuclease V was added , elect rophoresis was taken , CPD was measured by Gel Imaging and Image Analysis. It was found that antioxidant s exhibited some inhibitory effects on the formation of CPD ,β2carotene and superoxide dismutase ( SOD) are more obvious than GSH and CAT , but none of which were able to block the formation completely. The result s suggest that the formation of CPD is only partly dependent on ROS. Related Articles | Metrics

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STUDY ON THE ANEUPLOIDY FREQUENCY IN HUMAN SPERM CHROMOSOME USING FLUORESCENCE IN SITU HYBRIDIZATION ( FISH) Zheng Lukang , Deng Lixia , Zhang qiao Carcinogenesis, Teratogenesis & Mutagenesis    1998, 10 (5): 267-271.   DOI: 10.3969/j.issn.1004-616x.1998.05.001 Abstract （3066）      PDF （217KB）（3562）       Knowledge map    Save The f requency of 　Human sperm chromosome aneuploidy was assessed from 10 normal and health adult s by using the method of FISH. Sperm heads were decondensed with dithilthreitol (DTT) and lithium diiodosalicylate (LIS) , then the sperm nuclei were hybridized with biotin labeled alpha satellite X chromosome specific DNA probe (DXZ1) in 37 ℃ overnight . The hybridization signals were detected with Cy32St reptavidin. The red hybridization signals in X sperms were clearly visible under the Nikon fluorescence microscope. The hybridization efficiency was 48. 62 % ( rance 47. 54 % to 49. 36 %) . A total 55453 X sperm nuclei were observed. The frequency of disomic sperms was 0. 06 %. There was no significant difference observed in disomic frequency of X sperms between ours and that reported by former authors. The information provided by this paper is valuable for further studies on men exposed occupationally or environmentally to potential aneuploidogenic agents. Related Articles | Metrics

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Review; Carcinogenesis, Teratogenesis & Mutagenesis    2002, 14 (4): 249-252.   DOI: 10.3969/j.issn.1004-616x.2002.04.014 Abstract （2180）      PDF （84KB）（3561）       Knowledge map    Save Related Articles | Metrics