流产死胎组织中的巨细胞病毒、弓形虫感染监测

doi:10.3969/j.issn.1004-616x.1997.01.009

癌变·畸变·突变 ›› 1997, Vol. 09 ›› Issue (1): 28-31.doi: 10.3969/j.issn.1004-616x.1997.01.009

流产死胎组织中的巨细胞病毒、弓形虫感染监测

尚世强　洪文澜　石一复　余钟声　顾佩宝　潘存梅　俞锡林　郑兴宝

浙江医科大学　杭州　310003

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-01-30 发布日期:1997-01-30

DETECTION OF CYTOMEGALOVIRUS AND TOXOPLASMA GONDII INFECTION IN FETAL TISSUES OF ABORTION AND STILLBIRTHS

Shang Shiqiang, Hong Wenlan, Shi Yifu, etal

Zhejiang Medical U niversity, H angzhou, 310003

Received:1900-01-01 Revised:1900-01-01 Online:1997-01-30 Published:1997-01-30

摘要/Abstract

摘要： 本文通过人巨细胞病毒(HCMV)分离、套式聚合酶反应(PCR)及限制酶切分析、弓形虫分离及一次PCR,对不明原因的流产、死胎组织进行HCMV及弓形虫检测。结果发现,28 份流产、死胎组织中,1例死胎肺组织HCMV DNA 阳性, 病毒分离阴性;1例胎儿心、肺、脑、肝、肾、眼组织弓形虫DNA阳性,其脑、肺、眼、肝组织经动物接种后,在小白鼠腹水中找到弓形虫滋养体,但心、肾结果阴性。提示:临床上有些不明原因的流产、死胎是由于胎儿感染HCMV或弓形虫所致。PCR技术较常规病原体分离敏感、快速,适合于HCMV/弓形虫感染所致流产、死胎的早期诊断。

关键词: 巨细胞病毒, 弓形虫, 聚合酶链反应, 死胎

Abstract: Human cytomegalovirus (HCMV) and Toxoplasma gondii (Tox) were detected intissues of abortions and stillbirths Diagnosticmethods involved virus isolation, nested PCR,restriction endonucleases analyses of HCMV and the etiological isolation of Tox- trophozoites through m ice inoculation and single PCR to Tox The results showed, that among28 stillbirths and abortions HCMV DNA was found in the lung tissue of one fetal death ,which was negative in virus isolation. Tox DNA was positive in all tissues of a stillbirth including the heart, lungs, brain, liver, kidney ,and eye tissues Tox tachyziotes was isolated from the brain ,lung ,eye and liver tissues in the fetal death through animal inocuation but negative resultswere seen in the heart and kidney .A ll these suggests that some abortions and stillbirths result from fetal infection w ith HCMV or Tox, PCR, being more sensitive and rapid than pathogen isolation, m ight be useful for early diagnosis of abortions and stillbirths w ith HCMV or Tox infection.

Key words: Cytomegalovirus, Toxoplasma PCR, Stillbirth

尚世强　洪文澜　石一复　余钟声　顾佩宝　潘存梅　俞锡林　郑兴宝. 流产死胎组织中的巨细胞病毒、弓形虫感染监测[J]. 癌变·畸变·突变, 1997, 09(1): 28-31.

Shang Shiqiang, Hong Wenlan, Shi Yifu, etal. DETECTION OF CYTOMEGALOVIRUS AND TOXOPLASMA GONDII INFECTION IN FETAL TISSUES OF ABORTION AND STILLBIRTHS[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 1997, 09(1): 28-31.

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流产死胎组织中的巨细胞病毒、弓形虫感染监测

DETECTION OF CYTOMEGALOVIRUS AND TOXOPLASMA GONDII INFECTION IN FETAL TISSUES OF ABORTION AND STILLBIRTHS

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