癌变·畸变·突变 ›› 1998, Vol. 10 ›› Issue (1): 28-030.doi: 10.3969/j.issn.1004-616x.1998.01.009

• 论著 • 上一篇    下一篇

氟化物对不同类型DNA 紫外吸收光谱的影响

高希宝 　刘春生 　孙建霞 　张　宁　 许　之　 李　杰   

  1. 山东医科大学公共卫生学院　济南　250012
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1998-01-30 发布日期:1998-01-30

THE EFFECTS OF SODIUM FLUORIDE ON THE UV ABSORPTION SPECTRUM OF DIFFERENT TYPES OF DNA

Gao Xibao , Liu Chunsheng , Sun J ianxia , Zhang Ning , Xu Zhi , Li J ie   

  1. School of public heal th , S handong Medical University , Jinan 250012
  • Received:1900-01-01 Revised:1900-01-01 Online:1998-01-30 Published:1998-01-30

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摘要： 以紫外吸收光谱法测定了氟化钠(NaF) 与不同类型DNA 的直接结合作用。结果表明,NaF (0. 15 -23. 6mmol/ L) 可使线状小牛胸腺DNA(2214mg/ L) 及Lamds DNA 的紫外吸收光谱特征发生明显改变,表现为吸收波长的明显位移及吸收强度的显著降低;而10 —80mmol/ L NaF 未诱发环状PBR322质粒DNA 紫外吸收光谱特征发生改变。从而较好地解释了以往氟遗传毒性研究中,哺乳动物细胞染色体损伤及基因突变试验多为阳性,而细菌基因回复突变试验呈阴性的结果。

关键词: 氟化物, DNA 结合, 紫外光谱。

Abstract: The direct combination of sodium fluoride(NaF) to the different types of DNA was investigated using UV absorption spect romet ry. The result s showed that NaF (0. 15 - 23. 6mmol/L) could significantly change the UV absorption spect rum of the linar type fetal bovine thymus DNA (22. 4 mg/ L) and Lamds DNA(0. 9mg/ L) . It s absorption peak was shifted and declined.NaF (10 - 80 mmol/ L) could not change the UV absorption spect rum of the circular type PBR322DNA. These may explain the result s of that NaF induce clastogenicity and mutation in mammalian cell assays but not in bacteria mutation test s.

Key words: Fluoride, DNA combination, UV absorption spect rum.

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