癌变·畸变·突变 ›› 1999, Vol. 11 ›› Issue (4): 191-194.doi: 10.3969/j.issn.1004-616x.1999.04.010

• 论著 • 上一篇    下一篇

基因重组人碱性成纤维细胞生长因子( rh-bFGF) 致突变研究

马明福1 　李练兵1 　蔡　敏1 　李新生1 　曾维三1 　廖明阳2 　王治乔2   

  1. 1重应市计划生育科学研究所　重庆　400020 　2 军事医学科学院毒物药物研究　北京　100850
  • 收稿日期:1998-10-28 修回日期:1999-03-01 出版日期:1999-07-30 发布日期:1999-07-30

STUDY ON MUTAGENICITY ON RECOMBINANT HUMAN BASIC FIBROBLAST CROWTH FACTOR

Ma Mingfu1 , Li Lianbing1 , Cai Min1 , Li Xinsheng1 , Zeng Weisan1 , Liao Mingyang2 , Wang Zhiqiao2   

  1. 1Chongqing Family Planning Scientif ic Research Instit ute , Chongqing 　400020 　2 Instit ute of Pharmacology and Toxicology , Academy of Military Medical Sciences , Beijing 　100850
  • Received:1998-10-28 Revised:1999-03-01 Online:1999-07-30 Published:1999-07-30

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摘要： 本文用Ames 试验,体外染色体畸变试验和微核试验对rh2bFGF 进行了致突变性研究。结果显示,rh2bFGF 的各剂量组对TA97 、TA98 、TA100 、TA102菌株在±S9mix条件下无致突变性;在±S9mix条件下的染色体畸变试验中,rh2bFGF 的4 个剂量浓度对CHL 细胞染色体无畸变作用;以0. 53mg/ KG·bw、2. 13mg/ KG·bw、8. 50mg/ KG·bw、34. 0mg/ KG·bw 的rh2bFGF 对小鼠骨髓多染红细胞无诱发微核增加作用。实验结果表明,rh2bFGF 无致突变作用。

关键词: rh-bFGF, Ames 试验, 染色体畸变, 微核, 突变

Abstract: This paper reported the research result s of mutagenicity of rh2bFGF. The result s showed that rh -FGF at 5 doses of 0. 1 - 500μg/ plate did not induce positive mutation of st rains TA97 、TA98 、TA100 and TA10 with or without S9mix in the Ames Test . The rates of CHL Chromosome aberrations of rh - bFGF at 4 doses were negative range ( < 5 %) . The rates of micronuleus assay of polychromatic erythrocytes in mice of rh -bFGF at doses of 0. 53μg/ KG·bw ,2. 13μg/ KG·bw , 85μg/ KG·bw ,34μg/ KG·bw would not be to induce increase of it s micronuclei. It is proved that rh - bFGF would not be any mutagenicity in this experiment .

Key words: rh - bFGF, Ames test, Chromosome abcrration, Micronucleus, Mutation

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