镉金属硫蛋白对小鼠脾淋巴细胞DNA 链损伤和修复的体外实验研究

doi:10.3969/j.issn.1004-616x.2000.02.003

癌变·畸变·突变 ›› 2000, Vol. 12 ›› Issue (2): 72-075.doi: 10.3969/j.issn.1004-616x.2000.02.003

镉金属硫蛋白对小鼠脾淋巴细胞DNA 链损伤和修复的体外实验研究

林忠宁,董胜璋,蔡　颖,董书芸,余贵英,任铁玲

中山医科大学公共卫生学院环境卫生学教研室,广东　广州　510089

收稿日期:1999-08-04 修回日期:1999-11-20 出版日期:2000-04-30 发布日期:2000-04-30

IN VITRO STUDY ON THE DNA INJURIES AND REPAIR OF SPLENIC LYMPHOCYTE INDUCED BY CADMIUM- METALLOTHIONEIN

LIN Zhong-ning , DONG Sheng-zhang , CAI Ying , DONG Shu-yun , YU Gui-ying , REN Tie-ling

Department of Envi ronmental Health , S un Yat2sen University of Medical Sciences , Guangz hou 510089 ,China

Received:1999-08-04 Revised:1999-11-20 Online:2000-04-30 Published:2000-04-30

摘要/Abstract

摘要： 目的:为研究外源性镉金属硫蛋白(CdMT) 对淋巴细胞DNA 的损伤和修复作用。方法:采用单细胞电泳法(SCGE) 检测DNA 单链断裂(SSBs) ,观察在脱金属硫蛋白(Apo - MT) 和不同终浓度CdMT(0. 1μmol Cd2 + / L 、1. 0μmol Cd2 + / L 、10. 0μmolCd2 + / L 、20. 0μmol Cd2 + / L) 染毒作用30 分钟和60 分钟时DNA 链损伤,和去除受试物后30 分钟、60 分钟、120 分钟时DNA链损伤的修复。结果:DNA - SSBs 尾长在Apo - MT 作用未见增加;在中、高剂量CdMT 染毒30 和60 分钟时都明显增长。损伤后链修复DNA 迁移尾长,在中、高剂量CdMT 组都显著低于细胞染毒时,但明显高于对照和Apo - MT 组。结论:提示CdMT 直接作用可致脾淋巴细胞DNA 链的损伤,同时细胞存在对单链断裂的修复机制。

关键词: 金属硫蛋白, 镉, DNA 损伤和修复, 单细胞电泳

Abstract: Purpose : To study and compare DNA injuries and repair in BALB/ C mice splenic lymphocyte induced by exogenous cadmium2metallothionein (CdMT) . Methods :Single cell gel elect rophoresis ( SCGE) assay was sed to detect DNA single st rand breaks (DNA2SSBs) when lymphocyte were induced with different concent ration of CdMT for 30 or 60 minutes , and the ability of DNA repair after CdMT was removed for different times from 30 to 120 minutes respectively. Results : It was showed that the comet length of DNA-SSBs did not found change in Apo2MT group , while there were significant increasement of the comet length in mid2 or high-dose CdMT group at both exposure times. And the comet length of each DNA repair times (30 to 120 min) in mid2 or,high-dose CdMT group was significantly shorter than that of DNA injuries , but longer than that of cont rol and Apo2MT group. Conclusion : It is indicated that lymphocyte DNA2SSBs damage would be induced by CdMT exposed directly , and that there were repair activity for DNA2SSBs in spleen lymphocyte in vit ro.

Key words: metallothionein, cadmium, DNA injury and repair, single cell gel elect rophoresis

中图分类号:

R730. 3
X591

林忠宁, 董胜璋, 蔡　颖, 董书芸, 余贵英, 任铁玲. 镉金属硫蛋白对小鼠脾淋巴细胞DNA 链损伤和修复的体外实验研究[J]. 癌变·畸变·突变, 2000, 12(2): 72-075.

LIN Zhong-ning , DONG Sheng-zhang , CAI Ying , DONG Shu-yun , YU Gui-ying , REN Tie-ling. IN VITRO STUDY ON THE DNA INJURIES AND REPAIR OF SPLENIC LYMPHOCYTE INDUCED BY CADMIUM- METALLOTHIONEIN[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2000, 12(2): 72-075.

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镉金属硫蛋白对小鼠脾淋巴细胞DNA 链损伤和修复的体外实验研究

IN VITRO STUDY ON THE DNA INJURIES AND REPAIR OF SPLENIC LYMPHOCYTE INDUCED BY CADMIUM- METALLOTHIONEIN

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