癌变·畸变·突变 ›› 2001, Vol. 13 ›› Issue (3): 147-150.doi: 10.3969/j.issn.1004-616x.2001.03.004

• 论著 • 上一篇    下一篇

珍香胶囊对人食管癌的抗癌作用及其机制研究

许锦阶1, 徐小虎1, 王德昌2, 曾宪明3   

  1. 1. 汕头大学医学院病理学和病理生理学系,广东　汕头　515031; 2. 中国医学科学院肿瘤研究所药理室,北京　100073 ;3. 中山医科大学实验动物中心,广东　广州　510089
  • 收稿日期:2000-09-19 修回日期:2000-12-26 出版日期:2001-07-30 发布日期:2001-07-30

ANTITUMOR EFFECT AND MECHANISMS OF ZHENXIANG CAPSULE ON HUMAN ESOPHAGEAL CARCINOMA

XU Jin-jie1, XU Xiao-hu1, WANG De-chang2, ZEN G Xian-ming3   

  1. 1. Department of Pathology and Pathophysiology Shantou University Medical College , Shantou 515031 ,China ; 2. Department of Pharmacology , Institute Oncology , Chinese Academy of Medical Sciences , Beijing 100073 , China ; 3. Department of Experiment Animal Center , Sun Yat2sen University of Medical Sciences ,Guangzhou 510087 , China
  • Received:2000-09-19 Revised:2000-12-26 Online:2001-07-30 Published:2001-07-30

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摘要： 目的:探讨珍香胶囊对人食管癌的抗癌作用及其机制。方法:应用光镜、透射电镜和流式细胞仪,观察珍香胶囊诱导体外培养的人食管癌细胞株EC109 细胞凋亡的作用;应用人食管癌裸鼠移植瘤模型,观察珍香胶囊的抗癌作用。结果:在体外,经珍香胶囊处理的EC109 细胞出现典型的细胞凋亡特征。在体内,珍香胶囊3 个剂量4 800 m g/ (kg·bw) 、2 400 mg/ (kg·bw) 、1 200 mg/ (kg·bw) ]对裸鼠移植的人食管癌的抑瘤率分别为70. 80 %～75. 00 %( P < 0. 01) 、58. 33 %～64. 71 %( P < 0. 01) 、37. 04 %～45. 10 %( P < 0. 01) ,阳性对照平阳霉毒(15 mg/ (kg·bw) ) 对裸鼠移植的人食管癌的抑瘤率为51. 33 %～60. 78 %( P< 0. 01) 。结论:珍香胶囊在体外有诱导人食管癌细胞产生凋亡的作用,这可能是珍香胶囊抗食管癌作用的重要机制。在裸鼠可耐受的剂量下,珍香胶囊对裸鼠移植的人食管癌有明显抗癌作用,且随着剂量增加,其抗癌疗效增加。

关键词: 珍香胶囊, 食管癌, 动物疾病模型, 裸鼠

Abstract: Purpose : To investigate the antitumor effect and mechanisms of Zhenxiang capsule (ZXC) on human esophageal carcinoma. Methods : Apoptosis induced by ZXC in human esophageal carcinoma cell line EC109 was investigated by optical microscopy , transmission elect ron microscopy and flow cytomet ry. Antitumor effect of ZXC was observed on human esophageal carcinoma xenograft in nude mice. Results : After exposure to ZXC ,EC109 cells manifested typical apoptotic features. The tumor2inhibiting rates in the groups of ZXC 4 800 mg/(kg·bw) , 2 400 mg/ (kg·bw) and 1 200 mg/ (kg·bw) ranged f rom 70. 80 % to 75. 00 %( P < 0. 01) , 58. 33 % to 64. 71 %( P < 0. 01) and 37. 04 % to 45. 10 %( P < 0. 01) , respectively. The tumor2inhibiting rates of the pingyangmycin (15 mg/ (kg·bw) ) group ranged f rom 51. 33 % to 60. 78 %( P < 0. 01) . Conclusion : ZXC induces apoptosis of esophageal carcinoma cells in vit ro , which is possibly the primary mechamism of ZXC inhibition of esophageal carcinoma. ZXC has significant antitumor effect on human esophageal carcinoma t ransplanted into nude mice at tolerant doses. The antitumor effect increases with dosage.

Key words: zhenxiang capsule, esophageal carcinoma, animal disease model, nude mouse

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