癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (3): 141-143.doi: 10.3969/j.issn.1004-616x.2003.03.004

• 论著 • 上一篇    下一篇

Aroclor1254预先染毒增强苯并[a]芘对Hep G2细胞DNA的损伤

吴欣江1, 鲁文清1, Mersch-Sundermann V 2   

  1. 1.华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系， 湖北　武汉　430030； 2.Giessen大学医学院室内与环境毒理研究室， Giessen 35385， 德国
  • 收稿日期:2002-10-18 修回日期:2003-01-04 出版日期:2003-07-30 发布日期:2003-07-30
  • 通讯作者: 鲁文清

DNA DAMAGE INDUCED BY BENZO[a]PYRENE WAS MAGNIFIED AFTER PRE-TREATMENT OF AROCLOR1254 IN Hep G2 CELLS

Wu Xin-Jiang 1, Lu Wen-Qing 1, Mersch-Sundermann V 2   

  1. 1. Departement of Occupational and Environmental Health, Tongji Medical College, Huazhong University of Science and Techlology, Wuhan 430030, China; 2. Department of Indoor and Environmental Toxicology, Faculty of Medicine, University of Giessen Giessen 35385, Germany
  • Received:2002-10-18 Revised:2003-01-04 Online:2003-07-30 Published:2003-07-30
  • Contact: Lu Wen-Qing

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摘要： 目的： 探讨Hep G2细胞经Aroclor1254预处理后对苯并[a]芘诱发的DNA损伤的影响。 方法： 运用单细胞凝胶电泳技术检测了Hep G2细胞经Aroclor1254(23、46、92和184 μmol / L)单独染毒24 h和将其预处理24 h后再用苯并[a]芘染毒1 h对DNA损伤的影响。 结果： 苯并[a]芘诱发的DNA损伤随着Aroclor1254预处理的浓度增大而升高，呈明显的剂量效应关系。当Aroclor1254预处理的浓度分别为46、92和184 μmol / L时，苯并[a]芘诱发的DNA损伤与苯并[a]芘单独作用相比分别升高了8 ％、16 ％和160 ％。184 μmol / L的Aroclor1254预处理后，苯并[a]芘诱发的DNA损伤与苯并[a]芘单独作用相比有极显著性差异(P<0.01)。 结论： Aroclor1254可显著地增强苯并[a]芘在Hep G2细胞中诱导的DNA损伤，这种损伤的增强可能和Aroclor1254对Hep G2细胞CYP1A1的诱导有关。

关键词: Aroclor1254, 苯并[a]芘, 单细胞凝胶电泳

Abstract: Purpose: To investigate the change of magnitude of DNA damage induced by B [a]P(benzo [a]pyrene) in Hep G2 cells after pre-treatment with Aroclor1254. Methods: single-cell gel electrophoresis(SCGE) was performed to assay DNA damages caused by aroclor1254(23, 46, 92 and 184 μmol / L) for 24 h alone treatment and B[a] P for 1 h treatment after 24 h pretreatment with Aroclor1254 in Hep G2 cells.Results:DNA damagesinduced by B[a] P increased 8 ％, 16 ％ and 160 ％ respectively in a dose-response manner after pretreatment of Aroclor1254 at 46, 92 and 184 μmol / L compared to the B[a]P treatment alone. A statistically significant increase of DNA damage caused by B[a]P was observed at the concentration of 184 μmol / L of Aroclor1254 pretreatment(P<0.01). Conclusion: DNA damage induced by B[a]P can be enhanced by Aroclor1254, which may be a result from induction of CYP1A1 by Aroclor1254.

Key words: aroclor1254, benzo[a]pyrene, single-cell gel electrophoresis(SCGE)

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